Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23529 | 5' | -52 | NC_005260.1 | + | 129522 | 0.66 | 0.99512 |
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Target: 5'- aGCGGCuuaguugUCCAGUCUguccagGGAGCaCCGu -3' miRNA: 3'- -UGUCGca-----AGGUCAGAaa----CCUUGcGGU- -5' |
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| 23529 | 5' | -52 | NC_005260.1 | + | 118336 | 0.66 | 0.994347 |
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Target: 5'- -uGGUGUgucUCCGGUC---GGAACGUCAg -3' miRNA: 3'- ugUCGCA---AGGUCAGaaaCCUUGCGGU- -5' |
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| 23529 | 5' | -52 | NC_005260.1 | + | 167727 | 0.67 | 0.98888 |
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Target: 5'- -aAGCGUUCCAG-CUUccUGGGcgagGCGUCu -3' miRNA: 3'- ugUCGCAAGGUCaGAA--ACCU----UGCGGu -5' |
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| 23529 | 5' | -52 | NC_005260.1 | + | 47548 | 0.67 | 0.98579 |
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Target: 5'- --uGCGcUCCGGUCUggcgucuaGGAACGUCGa -3' miRNA: 3'- uguCGCaAGGUCAGAaa------CCUUGCGGU- -5' |
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| 23529 | 5' | -52 | NC_005260.1 | + | 16750 | 0.69 | 0.969688 |
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Target: 5'- cAUGGCGUUCCAaagacUGGAACGCUg -3' miRNA: 3'- -UGUCGCAAGGUcagaaACCUUGCGGu -5' |
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| 23529 | 5' | -52 | NC_005260.1 | + | 16490 | 1.08 | 0.010827 |
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Target: 5'- uACAGCGUUCCAGUCUUUGGAACGCCAu -3' miRNA: 3'- -UGUCGCAAGGUCAGAAACCUUGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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