Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23530 | 3' | -46.5 | NC_005260.1 | + | 49899 | 0.66 | 0.999999 |
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Target: 5'- aCCgGCGAUUCCua--CGUUAUacugAUACg -3' miRNA: 3'- -GGgCGCUAAGGuaacGCAAUAg---UAUG- -5' |
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| 23530 | 3' | -46.5 | NC_005260.1 | + | 166504 | 0.67 | 0.999989 |
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Target: 5'- aUCGcCGGUUCUAcUGUGUUgGUCAUAUa -3' miRNA: 3'- gGGC-GCUAAGGUaACGCAA-UAGUAUG- -5' |
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| 23530 | 3' | -46.5 | NC_005260.1 | + | 176279 | 0.67 | 0.999989 |
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Target: 5'- gCCaaaGCGGUUuaCCA--GCGUUAUCGUGa -3' miRNA: 3'- -GGg--CGCUAA--GGUaaCGCAAUAGUAUg -5' |
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| 23530 | 3' | -46.5 | NC_005260.1 | + | 90642 | 0.67 | 0.999971 |
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Target: 5'- aCCCaucCGGUUCCGUUGUGUuugcauuacucuaUGUUGUAUg -3' miRNA: 3'- -GGGc--GCUAAGGUAACGCA-------------AUAGUAUG- -5' |
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| 23530 | 3' | -46.5 | NC_005260.1 | + | 125252 | 0.69 | 0.999754 |
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Target: 5'- aUCGCGAUUCg--UGCGUUAUgAUGu -3' miRNA: 3'- gGGCGCUAAGguaACGCAAUAgUAUg -5' |
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| 23530 | 3' | -46.5 | NC_005260.1 | + | 178941 | 0.7 | 0.999511 |
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Target: 5'- aCCGCGAaaCCAcUUGgaGUUGUUAUACa -3' miRNA: 3'- gGGCGCUaaGGU-AACg-CAAUAGUAUG- -5' |
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| 23530 | 3' | -46.5 | NC_005260.1 | + | 100713 | 0.7 | 0.999393 |
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Target: 5'- aCCGCGAcggauaaCAUUGCcGUUAUCAUcaACg -3' miRNA: 3'- gGGCGCUaag----GUAACG-CAAUAGUA--UG- -5' |
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| 23530 | 3' | -46.5 | NC_005260.1 | + | 49609 | 0.95 | 0.224908 |
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Target: 5'- aCCCGCGAUUCCAUUGCGUUcuaAUACa -3' miRNA: 3'- -GGGCGCUAAGGUAACGCAAuagUAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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