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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23530 | 5' | -48 | NC_005260.1 | + | 130278 | 0.66 | 0.999952 |
Target: 5'- ---aAUGGCGUAGuGAAUCGCgaugcUGGUAUu -3' miRNA: 3'- gucaUAUUGCAUC-CUUAGCG-----GCCAUG- -5' |
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23530 | 5' | -48 | NC_005260.1 | + | 33037 | 0.68 | 0.999292 |
Target: 5'- uCGGUAUAGCGauuaAAUCGCCaauGGUGCg -3' miRNA: 3'- -GUCAUAUUGCauccUUAGCGG---CCAUG- -5' |
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23530 | 5' | -48 | NC_005260.1 | + | 49643 | 1.11 | 0.019808 |
Target: 5'- uCAGUAUAACGUAGGAAUCGCCGGUACg -3' miRNA: 3'- -GUCAUAUUGCAUCCUUAGCGGCCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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