miRNA: 3'- -UGGUuauauUGUACCaGGGUCUa--UCU- -5'
miRNA: 3'- -UGGUuaua-UUGUACCA--GGGUCUAUCU- -5'
miRNA: 3'- uGGUUAUAUUGUACc-AGGGUCUAucu -5'
miRNA: 3'- -UGGUu--AUAUuguACCAGGGUCUAUcu -5'
miRNA: 3'- -UGGUUAUAUUGUACCAGGGUCUAUCU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23531 | 5' | -45.7 | NC_005260.1 | + | 179429 | 0.66 | 0.999999 |
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Target: 5'- uACCGu----GCAUGG-CCCAGAacgGGAc -3' miRNA: 3'- -UGGUuauauUGUACCaGGGUCUa--UCU- -5' |
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| 23531 | 5' | -45.7 | NC_005260.1 | + | 226263 | 0.68 | 0.99996 |
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Target: 5'- uGCUAuugaaAACAUGGUauUCCAGAUGGGa -3' miRNA: 3'- -UGGUuaua-UUGUACCA--GGGUCUAUCU- -5' |
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| 23531 | 5' | -45.7 | NC_005260.1 | + | 57117 | 0.69 | 0.999928 |
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Target: 5'- cCCAGUAUGACAUGcaUUCCAGAUu-- -3' miRNA: 3'- uGGUUAUAUUGUACc-AGGGUCUAucu -5' |
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| 23531 | 5' | -45.7 | NC_005260.1 | + | 52630 | 0.72 | 0.996952 |
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Target: 5'- gACCAuguUAUAu--UGGUCCCAGAUAc- -3' miRNA: 3'- -UGGUu--AUAUuguACCAGGGUCUAUcu -5' |
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| 23531 | 5' | -45.7 | NC_005260.1 | + | 52381 | 1.11 | 0.032632 |
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Target: 5'- gACCAAUAUAACAUGGUCCCAGAUAGAg -3' miRNA: 3'- -UGGUUAUAUUGUACCAGGGUCUAUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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