Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23532 | 5' | -47 | NC_005260.1 | + | 139887 | 0.66 | 0.999987 |
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Target: 5'- uGAAAUaCUCACuGAUCcgcgcuUGGACUCAg -3' miRNA: 3'- gCUUUAgGAGUGcCUAGu-----ACUUGAGU- -5' |
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| 23532 | 5' | -47 | NC_005260.1 | + | 203004 | 0.67 | 0.999935 |
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Target: 5'- gGAAAUCCUCACaaagacgGAACUCGa -3' miRNA: 3'- gCUUUAGGAGUGccuaguaCUUGAGU- -5' |
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| 23532 | 5' | -47 | NC_005260.1 | + | 113303 | 0.68 | 0.999869 |
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Target: 5'- gCGAAAcUUUCGCGGAUCuuuuuGUGcGACUCAa -3' miRNA: 3'- -GCUUUaGGAGUGCCUAG-----UAC-UUGAGU- -5' |
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| 23532 | 5' | -47 | NC_005260.1 | + | 96072 | 0.68 | 0.999869 |
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Target: 5'- aGAAGUUaUCGCGacucguccagcaGAUCAUGAAUUCAa -3' miRNA: 3'- gCUUUAGgAGUGC------------CUAGUACUUGAGU- -5' |
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| 23532 | 5' | -47 | NC_005260.1 | + | 130765 | 0.7 | 0.999334 |
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Target: 5'- uGuuAUCCUCAacCGGAguugCAUGaAGCUCGa -3' miRNA: 3'- gCuuUAGGAGU--GCCUa---GUAC-UUGAGU- -5' |
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| 23532 | 5' | -47 | NC_005260.1 | + | 57846 | 1.1 | 0.031641 |
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Target: 5'- uCGAAAUCCUCACGGAUCAUGAACUCAc -3' miRNA: 3'- -GCUUUAGGAGUGCCUAGUACUUGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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