Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23534 | 5' | -47.7 | NC_005260.1 | + | 176660 | 0.66 | 0.999969 |
Target: 5'- -cGCCAAUGcCGAUUuCGAGAU--CUCg -3' miRNA: 3'- gaCGGUUGC-GCUAAcGUUCUAauGAG- -5' |
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23534 | 5' | -47.7 | NC_005260.1 | + | 191284 | 0.66 | 0.999944 |
Target: 5'- -cGCCuuCGCuGUUGCGAGAgUUGC-Ca -3' miRNA: 3'- gaCGGuuGCGcUAACGUUCU-AAUGaG- -5' |
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23534 | 5' | -47.7 | NC_005260.1 | + | 193534 | 0.66 | 0.999925 |
Target: 5'- aUGCCAACGCGGa-GCA---UUGCUUc -3' miRNA: 3'- gACGGUUGCGCUaaCGUucuAAUGAG- -5' |
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23534 | 5' | -47.7 | NC_005260.1 | + | 125624 | 0.66 | 0.999925 |
Target: 5'- -cGCCAAUGCGA--GCAAa--UGCUCu -3' miRNA: 3'- gaCGGUUGCGCUaaCGUUcuaAUGAG- -5' |
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23534 | 5' | -47.7 | NC_005260.1 | + | 205612 | 0.67 | 0.999873 |
Target: 5'- -cGCCAcaACGCGGUUGguGucguuuGAcgUACUCa -3' miRNA: 3'- gaCGGU--UGCGCUAACguU------CUa-AUGAG- -5' |
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23534 | 5' | -47.7 | NC_005260.1 | + | 204822 | 0.67 | 0.99966 |
Target: 5'- uCUGCaCGGCGgcCGAUaucGCAAGAUUGCa- -3' miRNA: 3'- -GACG-GUUGC--GCUAa--CGUUCUAAUGag -5' |
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23534 | 5' | -47.7 | NC_005260.1 | + | 6053 | 0.67 | 0.999644 |
Target: 5'- uCUGCCAACGCu-UUGCcaucuuuguuguAGAUUACg- -3' miRNA: 3'- -GACGGUUGCGcuAACGu-----------UCUAAUGag -5' |
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23534 | 5' | -47.7 | NC_005260.1 | + | 154528 | 0.68 | 0.999572 |
Target: 5'- -gGCCAACuaauAUUGcCGAGAUUGCUCu -3' miRNA: 3'- gaCGGUUGcgc-UAAC-GUUCUAAUGAG- -5' |
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23534 | 5' | -47.7 | NC_005260.1 | + | 24702 | 0.68 | 0.999184 |
Target: 5'- gUGCCAAUGCGGUaGCA-GAUaAUUUa -3' miRNA: 3'- gACGGUUGCGCUAaCGUuCUAaUGAG- -5' |
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23534 | 5' | -47.7 | NC_005260.1 | + | 43270 | 0.69 | 0.998523 |
Target: 5'- aCUGUCGgcaGCGCGAUuucgaUGCAcAGAUUACcCa -3' miRNA: 3'- -GACGGU---UGCGCUA-----ACGU-UCUAAUGaG- -5' |
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23534 | 5' | -47.7 | NC_005260.1 | + | 176407 | 0.69 | 0.99822 |
Target: 5'- -gGUCAGCGCGAUUGUccGAaagACUUc -3' miRNA: 3'- gaCGGUUGCGCUAACGuuCUaa-UGAG- -5' |
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23534 | 5' | -47.7 | NC_005260.1 | + | 173811 | 0.69 | 0.997867 |
Target: 5'- -aGCCGcuuaACGCGAUUGCuaccguugcuGAGAUUAUa- -3' miRNA: 3'- gaCGGU----UGCGCUAACG----------UUCUAAUGag -5' |
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23534 | 5' | -47.7 | NC_005260.1 | + | 4884 | 0.7 | 0.996983 |
Target: 5'- -aGCCAGCGCGAgcGCGAGuccaaGCa- -3' miRNA: 3'- gaCGGUUGCGCUaaCGUUCuaa--UGag -5' |
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23534 | 5' | -47.7 | NC_005260.1 | + | 87918 | 0.71 | 0.992372 |
Target: 5'- aCUGCCAuuucguuuACGCGAUuugGgAAGAUaACUCc -3' miRNA: 3'- -GACGGU--------UGCGCUAa--CgUUCUAaUGAG- -5' |
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23534 | 5' | -47.7 | NC_005260.1 | + | 67041 | 1.11 | 0.020026 |
Target: 5'- aCUGCCAACGCGAUUGCAAGAUUACUCc -3' miRNA: 3'- -GACGGUUGCGCUAACGUUCUAAUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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