Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23535 | 5' | -42.5 | NC_005260.1 | + | 198601 | 0.68 | 1 |
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Target: 5'- cGAAUgUUGUCgGUAAACucuuuGAGCUUGAUc -3' miRNA: 3'- uCUUG-AACAGgCAUUUG-----UUUGAACUA- -5' |
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| 23535 | 5' | -42.5 | NC_005260.1 | + | 201919 | 0.66 | 1 |
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Target: 5'- aAGAACUUGUCCaucGGAC-GGCUcaUGAUa -3' miRNA: 3'- -UCUUGAACAGGca-UUUGuUUGA--ACUA- -5' |
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| 23535 | 5' | -42.5 | NC_005260.1 | + | 227541 | 0.68 | 1 |
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Target: 5'- aAGGGCUUGcUCCGaacuggGAACAAACU-GAc -3' miRNA: 3'- -UCUUGAAC-AGGCa-----UUUGUUUGAaCUa -5' |
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| 23535 | 5' | -42.5 | NC_005260.1 | + | 221613 | 0.67 | 1 |
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Target: 5'- uAGAugUUGuUCCGUccGGCGAACUcGAa -3' miRNA: 3'- -UCUugAAC-AGGCAu-UUGUUUGAaCUa -5' |
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| 23535 | 5' | -42.5 | NC_005260.1 | + | 191835 | 0.71 | 0.999995 |
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Target: 5'- cGGAGCUguUGUCCGUc-AUAAACUUGu- -3' miRNA: 3'- -UCUUGA--ACAGGCAuuUGUUUGAACua -5' |
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| 23535 | 5' | -42.5 | NC_005260.1 | + | 53764 | 0.71 | 0.999989 |
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Target: 5'- cGGAGCUUGUCCGUugcccaucauaguGAaguuaGCAGguACUUGAc -3' miRNA: 3'- -UCUUGAACAGGCA-------------UU-----UGUU--UGAACUa -5' |
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| 23535 | 5' | -42.5 | NC_005260.1 | + | 87291 | 1.07 | 0.112973 |
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Target: 5'- aAGAACUUGUCCGUAAACAAACUUGAUu -3' miRNA: 3'- -UCUUGAACAGGCAUUUGUUUGAACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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