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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
23539 | 3' | -49.2 | NC_005260.1 | + | 125982 | 0.66 | 0.999464 |
Target: 5'- gCAGCGg--UAGUUCUGGcAGCGGugcaaugguuGGUCc -3' miRNA: 3'- -GUCGUauaAUCGAGAUU-UCGCC----------CCAG- -5' |
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23539 | 3' | -49.2 | NC_005260.1 | + | 147259 | 0.66 | 0.999182 |
Target: 5'- aAGCAUGUUcgcccAGCUCgaaaccGGAGCuGGGUa -3' miRNA: 3'- gUCGUAUAA-----UCGAGa-----UUUCGcCCCAg -5' |
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23539 | 3' | -49.2 | NC_005260.1 | + | 46637 | 0.68 | 0.996458 |
Target: 5'- uGGCAUAUgucguauugucGGCUCaAAGGCGGuGGUg -3' miRNA: 3'- gUCGUAUAa----------UCGAGaUUUCGCC-CCAg -5' |
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23539 | 3' | -49.2 | NC_005260.1 | + | 179169 | 0.68 | 0.996458 |
Target: 5'- aAGCAUAUgacgauGCUCUGuuGU-GGGUCa -3' miRNA: 3'- gUCGUAUAau----CGAGAUuuCGcCCCAG- -5' |
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23539 | 3' | -49.2 | NC_005260.1 | + | 139241 | 1.12 | 0.011314 |
Target: 5'- aCAGCAUAUUAGCUCUAAAGCGGGGUCg -3' miRNA: 3'- -GUCGUAUAAUCGAGAUUUCGCCCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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