Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23539 | 5' | -55.6 | NC_005260.1 | + | 53928 | 0.66 | 0.95389 |
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Target: 5'- aCGA-UCUGCAUCUGCUuUCUGUUUUa -3' miRNA: 3'- aGCUgGGGCGUAGACGA-GGACGAAAa -5' |
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| 23539 | 5' | -55.6 | NC_005260.1 | + | 228638 | 0.66 | 0.936404 |
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Target: 5'- aUCGGCCCuCGCGcUCUGa--CUGCUa-- -3' miRNA: 3'- -AGCUGGG-GCGU-AGACgagGACGAaaa -5' |
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| 23539 | 5' | -55.6 | NC_005260.1 | + | 89293 | 0.67 | 0.931452 |
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Target: 5'- aCGAgCCUGUAgCUGUUCCUGUUc-- -3' miRNA: 3'- aGCUgGGGCGUaGACGAGGACGAaaa -5' |
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| 23539 | 5' | -55.6 | NC_005260.1 | + | 4397 | 0.67 | 0.903171 |
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Target: 5'- aUUGACCCgauuauCGCAUCUGCaCUUGCg--- -3' miRNA: 3'- -AGCUGGG------GCGUAGACGaGGACGaaaa -5' |
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| 23539 | 5' | -55.6 | NC_005260.1 | + | 163129 | 0.68 | 0.869177 |
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Target: 5'- aUGGCUuaugCCGUAUCUGCgguggCCUGCUa-- -3' miRNA: 3'- aGCUGG----GGCGUAGACGa----GGACGAaaa -5' |
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| 23539 | 5' | -55.6 | NC_005260.1 | + | 211273 | 0.71 | 0.739341 |
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Target: 5'- cUGugCCUGCGUUaGCUUCUGCUUg- -3' miRNA: 3'- aGCugGGGCGUAGaCGAGGACGAAaa -5' |
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| 23539 | 5' | -55.6 | NC_005260.1 | + | 139275 | 1.06 | 0.006004 |
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Target: 5'- uUCGACCCCGCAUCUGCUCCUGCUUUUg -3' miRNA: 3'- -AGCUGGGGCGUAGACGAGGACGAAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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