Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2354 | 3' | -54 | NC_001416.1 | + | 11118 | 0.66 | 0.737284 |
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Target: 5'- aAGCGGuGAGGCUCagauuGCGUCCaUCAgCCAg -3' miRNA: 3'- -UCGUC-CUUUGAG-----UGCGGGaAGU-GGUg -5' |
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| 2354 | 3' | -54 | NC_001416.1 | + | 16697 | 0.66 | 0.735116 |
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Target: 5'- uGUAGcGGAACggCGCGCCaucauccggcaUCACCACa -3' miRNA: 3'- uCGUC-CUUUGa-GUGCGGga---------AGUGGUG- -5' |
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| 2354 | 3' | -54 | NC_001416.1 | + | 24819 | 0.67 | 0.636783 |
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Target: 5'- aGGCAGGAAGacaaaCACagaGCUCUUCAUCAUu -3' miRNA: 3'- -UCGUCCUUUga---GUG---CGGGAAGUGGUG- -5' |
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| 2354 | 3' | -54 | NC_001416.1 | + | 20486 | 0.67 | 0.636783 |
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Target: 5'- cAGCAGcGGAACggaGCGCCuCU--GCCGCg -3' miRNA: 3'- -UCGUC-CUUUGag-UGCGG-GAagUGGUG- -5' |
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| 2354 | 3' | -54 | NC_001416.1 | + | 2570 | 0.68 | 0.59149 |
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Target: 5'- cAGCGGGAAauACUgAUGCgCagCGCCGCc -3' miRNA: 3'- -UCGUCCUU--UGAgUGCGgGaaGUGGUG- -5' |
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| 2354 | 3' | -54 | NC_001416.1 | + | 6979 | 0.68 | 0.569022 |
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Target: 5'- cGGCAGGAAGUUCuuuuugACGCCgUUUuCCACg -3' miRNA: 3'- -UCGUCCUUUGAG------UGCGGgAAGuGGUG- -5' |
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| 2354 | 3' | -54 | NC_001416.1 | + | 9536 | 0.7 | 0.503182 |
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Target: 5'- cAGCGuGGu-GCUC-UGCCCUuUCACCACc -3' miRNA: 3'- -UCGU-CCuuUGAGuGCGGGA-AGUGGUG- -5' |
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| 2354 | 3' | -54 | NC_001416.1 | + | 30936 | 0.76 | 0.211594 |
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Target: 5'- cAGCAGGAAA---ACGCCCUUCGCaGCa -3' miRNA: 3'- -UCGUCCUUUgagUGCGGGAAGUGgUG- -5' |
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| 2354 | 3' | -54 | NC_001416.1 | + | 17497 | 1.09 | 0.000982 |
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Target: 5'- gAGCAGGAAACUCACGCCCUUCACCACc -3' miRNA: 3'- -UCGUCCUUUGAGUGCGGGAAGUGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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