miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23542 5' -47.6 NC_005260.1 + 94326 0.66 0.999946
Target:  5'- --aUCUCCGCUaUGuCGUUCUUUgUGUg -3'
miRNA:   3'- cugAGAGGCGAaACuGUAAGGAA-ACA- -5'
23542 5' -47.6 NC_005260.1 + 168297 0.67 0.999906
Target:  5'- gGGCUCUCCGCUUUuAUuuuUUCCa---- -3'
miRNA:   3'- -CUGAGAGGCGAAAcUGu--AAGGaaaca -5'
23542 5' -47.6 NC_005260.1 + 182580 0.71 0.994511
Target:  5'- uGAUUCUUgGUguuagaaaucaaggUGACAUUCCUUUGUa -3'
miRNA:   3'- -CUGAGAGgCGaa------------ACUGUAAGGAAACA- -5'
23542 5' -47.6 NC_005260.1 + 168070 1.09 0.025403
Target:  5'- aGACUCUCCGCUUUGACAUUCCUUUGUa -3'
miRNA:   3'- -CUGAGAGGCGAAACUGUAAGGAAACA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.