Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23542 | 5' | -47.6 | NC_005260.1 | + | 94326 | 0.66 | 0.999946 |
Target: 5'- --aUCUCCGCUaUGuCGUUCUUUgUGUg -3' miRNA: 3'- cugAGAGGCGAaACuGUAAGGAA-ACA- -5' |
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23542 | 5' | -47.6 | NC_005260.1 | + | 168297 | 0.67 | 0.999906 |
Target: 5'- gGGCUCUCCGCUUUuAUuuuUUCCa---- -3' miRNA: 3'- -CUGAGAGGCGAAAcUGu--AAGGaaaca -5' |
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23542 | 5' | -47.6 | NC_005260.1 | + | 182580 | 0.71 | 0.994511 |
Target: 5'- uGAUUCUUgGUguuagaaaucaaggUGACAUUCCUUUGUa -3' miRNA: 3'- -CUGAGAGgCGaa------------ACUGUAAGGAAACA- -5' |
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23542 | 5' | -47.6 | NC_005260.1 | + | 168070 | 1.09 | 0.025403 |
Target: 5'- aGACUCUCCGCUUUGACAUUCCUUUGUa -3' miRNA: 3'- -CUGAGAGGCGAAACUGUAAGGAAACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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