miRNA: 3'- guaaAGCCGUUgaca---UUAGACUGGUa -5'
miRNA: 3'- guaaAGCCGUuGACAuUAGACUGgua -5'
miRNA: 3'- guaAAGCCGUUGAC-AUUAGAcuGGua -5'
miRNA: 3'- -GUAAAGCCGUUGACAUUAGACUGGUA- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23544 | 3' | -46.5 | NC_005260.1 | + | 220011 | 0.66 | 0.999995 |
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Target: 5'- ---gUCGGCAAaauaaacAAUCUGACCGg -3' miRNA: 3'- guaaAGCCGUUgaca---UUAGACUGGUa -5' |
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| 23544 | 3' | -46.5 | NC_005260.1 | + | 227224 | 0.66 | 0.999991 |
|
Target: 5'- ---aUCGGCAuCUGUcAUCUGAUa-- -3' miRNA: 3'- guaaAGCCGUuGACAuUAGACUGgua -5' |
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| 23544 | 3' | -46.5 | NC_005260.1 | + | 224018 | 0.67 | 0.999954 |
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Target: 5'- --gUUCGGCAGCUGaUAAUCUu-CCu- -3' miRNA: 3'- guaAAGCCGUUGAC-AUUAGAcuGGua -5' |
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| 23544 | 3' | -46.5 | NC_005260.1 | + | 183854 | 1.07 | 0.041286 |
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Target: 5'- gCAUUUCGGCAACUGUAAUCUGACCAUg -3' miRNA: 3'- -GUAAAGCCGUUGACAUUAGACUGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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