Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23544 | 5' | -50.4 | NC_005260.1 | + | 228494 | 0.66 | 0.99888 |
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Target: 5'- ---cGUCAAGAUGCagaucGCGCCGGuCGUa- -3' miRNA: 3'- guuaCAGUUCUGUG-----UGCGGCU-GCAgu -5' |
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| 23544 | 5' | -50.4 | NC_005260.1 | + | 99532 | 0.66 | 0.998649 |
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Target: 5'- aGAUGaUCAAGGCACuGCGuuGGUGUCc -3' miRNA: 3'- gUUAC-AGUUCUGUG-UGCggCUGCAGu -5' |
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| 23544 | 5' | -50.4 | NC_005260.1 | + | 81000 | 0.67 | 0.998378 |
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Target: 5'- --cUGgagCAGGGCAUuaccguCGCCGACGUa- -3' miRNA: 3'- guuACa--GUUCUGUGu-----GCGGCUGCAgu -5' |
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| 23544 | 5' | -50.4 | NC_005260.1 | + | 176297 | 0.67 | 0.998062 |
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Target: 5'- -----gUAAGcACACugGCCGACGcCAa -3' miRNA: 3'- guuacaGUUC-UGUGugCGGCUGCaGU- -5' |
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| 23544 | 5' | -50.4 | NC_005260.1 | + | 198351 | 0.68 | 0.994899 |
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Target: 5'- ---gGUCAAGaAUACGCGCUGGC-UCGu -3' miRNA: 3'- guuaCAGUUC-UGUGUGCGGCUGcAGU- -5' |
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| 23544 | 5' | -50.4 | NC_005260.1 | + | 156055 | 0.68 | 0.993195 |
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Target: 5'- aGAUGUUGAGAUucuggaACGCCGA-GUCAa -3' miRNA: 3'- gUUACAGUUCUGug----UGCGGCUgCAGU- -5' |
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| 23544 | 5' | -50.4 | NC_005260.1 | + | 16046 | 0.72 | 0.941981 |
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Target: 5'- ---aGUCAAGcaACGgGCGuuGACGUCAg -3' miRNA: 3'- guuaCAGUUC--UGUgUGCggCUGCAGU- -5' |
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| 23544 | 5' | -50.4 | NC_005260.1 | + | 122113 | 0.76 | 0.793883 |
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Target: 5'- ---cGUCGAGAaacuuaACGCCGACGUCGa -3' miRNA: 3'- guuaCAGUUCUgug---UGCGGCUGCAGU- -5' |
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| 23544 | 5' | -50.4 | NC_005260.1 | + | 183890 | 1.09 | 0.016619 |
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Target: 5'- gCAAUGUCAAGACACACGCCGACGUCAc -3' miRNA: 3'- -GUUACAGUUCUGUGUGCGGCUGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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