Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23545 | 3' | -51.8 | NC_005260.1 | + | 231759 | 0.66 | 0.996511 |
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Target: 5'- aGACCGCGAGaacagGUUGCGgagcucucguCGCAcuuagAGCg -3' miRNA: 3'- -CUGGCGCUCagaa-CAAUGC----------GCGU-----UCG- -5' |
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| 23545 | 3' | -51.8 | NC_005260.1 | + | 181059 | 0.66 | 0.994509 |
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Target: 5'- -uCCGCGuGUUggauauCGCGCAAGUg -3' miRNA: 3'- cuGGCGCuCAGaacaauGCGCGUUCG- -5' |
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| 23545 | 3' | -51.8 | NC_005260.1 | + | 219286 | 0.67 | 0.993665 |
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Target: 5'- uAUCGCcAGUCUUGUugacUGCGgaGCAGGUa -3' miRNA: 3'- cUGGCGcUCAGAACA----AUGCg-CGUUCG- -5' |
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| 23545 | 3' | -51.8 | NC_005260.1 | + | 89488 | 0.68 | 0.984469 |
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Target: 5'- gGACCGUGAGUauguaUGUuaaaUAUGCGCGucuuGCu -3' miRNA: 3'- -CUGGCGCUCAga---ACA----AUGCGCGUu---CG- -5' |
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| 23545 | 3' | -51.8 | NC_005260.1 | + | 118371 | 0.68 | 0.984469 |
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Target: 5'- aACCGCGAGUggUGcaUUugGUcaaaugGCAAGCg -3' miRNA: 3'- cUGGCGCUCAgaAC--AAugCG------CGUUCG- -5' |
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| 23545 | 3' | -51.8 | NC_005260.1 | + | 157442 | 0.68 | 0.982587 |
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Target: 5'- ---aGCGGGUCaacGUUA-GCGCGAGCa -3' miRNA: 3'- cuggCGCUCAGaa-CAAUgCGCGUUCG- -5' |
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| 23545 | 3' | -51.8 | NC_005260.1 | + | 59619 | 0.69 | 0.970516 |
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Target: 5'- cGCCGCGAGUUaUGUUAuUGCcaacuaCAAGCa -3' miRNA: 3'- cUGGCGCUCAGaACAAU-GCGc-----GUUCG- -5' |
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| 23545 | 3' | -51.8 | NC_005260.1 | + | 67503 | 0.7 | 0.963308 |
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Target: 5'- uGACCGUGAGUgCUccgguaucucguccUGUUAC-UGUAAGCa -3' miRNA: 3'- -CUGGCGCUCA-GA--------------ACAAUGcGCGUUCG- -5' |
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| 23545 | 3' | -51.8 | NC_005260.1 | + | 185235 | 1.13 | 0.00648 |
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Target: 5'- gGACCGCGAGUCUUGUUACGCGCAAGCa -3' miRNA: 3'- -CUGGCGCUCAGAACAAUGCGCGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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