Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23548 | 3' | -44.9 | NC_005260.1 | + | 147221 | 0.67 | 1 |
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Target: 5'- cCCAUUuuaauGGGGuAAUCGauguuuGCACGUAAUCa -3' miRNA: 3'- -GGUAGc----CCUCuUUAGU------UGUGCAUUAG- -5' |
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| 23548 | 3' | -44.9 | NC_005260.1 | + | 198180 | 0.66 | 1 |
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Target: 5'- uUCGUCGuGAGuAGAUCccacGACaACGUAAUCg -3' miRNA: 3'- -GGUAGCcCUC-UUUAG----UUG-UGCAUUAG- -5' |
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| 23548 | 3' | -44.9 | NC_005260.1 | + | 77467 | 0.67 | 1 |
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Target: 5'- -aGUCGGuGAuGAAGUCAGCAUGa---- -3' miRNA: 3'- ggUAGCC-CU-CUUUAGUUGUGCauuag -5' |
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| 23548 | 3' | -44.9 | NC_005260.1 | + | 192890 | 0.66 | 1 |
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Target: 5'- uCCggCGGGAuuuuucuauugGAAAUCAACAUGaUAAa- -3' miRNA: 3'- -GGuaGCCCU-----------CUUUAGUUGUGC-AUUag -5' |
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| 23548 | 3' | -44.9 | NC_005260.1 | + | 26853 | 0.66 | 1 |
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Target: 5'- -aGUCGGGGGAGGU-AAUACGcuugGAUUa -3' miRNA: 3'- ggUAGCCCUCUUUAgUUGUGCa---UUAG- -5' |
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| 23548 | 3' | -44.9 | NC_005260.1 | + | 226082 | 0.71 | 0.999789 |
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Target: 5'- aCAU-GGGAGAAAUCAAU-CGUGAa- -3' miRNA: 3'- gGUAgCCCUCUUUAGUUGuGCAUUag -5' |
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| 23548 | 3' | -44.9 | NC_005260.1 | + | 92258 | 0.72 | 0.999466 |
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Target: 5'- gCAUCGGGAGuGAUCAACAg------ -3' miRNA: 3'- gGUAGCCCUCuUUAGUUGUgcauuag -5' |
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| 23548 | 3' | -44.9 | NC_005260.1 | + | 134749 | 0.76 | 0.988546 |
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Target: 5'- uCCAUCGcgcuGGGGuuAUCAGCACGUAu-- -3' miRNA: 3'- -GGUAGC----CCUCuuUAGUUGUGCAUuag -5' |
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| 23548 | 3' | -44.9 | NC_005260.1 | + | 200846 | 1.16 | 0.025925 |
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Target: 5'- aCCAUCGGGAGAAAUCAACACGUAAUCg -3' miRNA: 3'- -GGUAGCCCUCUUUAGUUGUGCAUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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