Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23549 | 5' | -48.9 | NC_005260.1 | + | 184217 | 0.66 | 0.999783 |
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Target: 5'- aAGAauaUUCAUCGggUCuAGUUUUGCUGu -3' miRNA: 3'- -UCUg--AAGUAGCuaGGuUCGAAACGGC- -5' |
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| 23549 | 5' | -48.9 | NC_005260.1 | + | 188072 | 0.66 | 0.999725 |
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Target: 5'- cGACUUUGUUGAUCUuauGCUUgugUGCUa -3' miRNA: 3'- uCUGAAGUAGCUAGGuu-CGAA---ACGGc -5' |
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| 23549 | 5' | -48.9 | NC_005260.1 | + | 167510 | 0.66 | 0.999725 |
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Target: 5'- cGAUaUCGUUGGUCCAcauagagaAGUUggUGCCGu -3' miRNA: 3'- uCUGaAGUAGCUAGGU--------UCGAa-ACGGC- -5' |
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| 23549 | 5' | -48.9 | NC_005260.1 | + | 117512 | 0.67 | 0.99946 |
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Target: 5'- cGGCUUuuugcCGUCGAUaUCAGGCUuggguuuuUUGCCGu -3' miRNA: 3'- uCUGAA-----GUAGCUA-GGUUCGA--------AACGGC- -5' |
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| 23549 | 5' | -48.9 | NC_005260.1 | + | 190046 | 0.7 | 0.988883 |
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Target: 5'- gAGGCUUCGUCcuuGAacuUgUAAGUUUUGCCGa -3' miRNA: 3'- -UCUGAAGUAG---CU---AgGUUCGAAACGGC- -5' |
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| 23549 | 5' | -48.9 | NC_005260.1 | + | 201443 | 1.09 | 0.01958 |
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Target: 5'- uAGACUUCAUCGAUCCAAGCUUUGCCGu -3' miRNA: 3'- -UCUGAAGUAGCUAGGUUCGAAACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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