Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23550 | 5' | -54.5 | NC_005260.1 | + | 232222 | 0.66 | 0.975283 |
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Target: 5'- -cGGCAGUGaaagucuuGCCGUCUGCCAUg -3' miRNA: 3'- caUUGUCGCcccuu---UGGCAGACGGUA- -5' |
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| 23550 | 5' | -54.5 | NC_005260.1 | + | 551 | 0.67 | 0.957168 |
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Target: 5'- -aAugAGCGuuGGAucAACCGUCUGUCAUc -3' miRNA: 3'- caUugUCGCc-CCU--UUGGCAGACGGUA- -5' |
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| 23550 | 5' | -54.5 | NC_005260.1 | + | 70245 | 0.68 | 0.935592 |
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Target: 5'- -gAACAGCGuGGAacgAACUGUCUuGCCGg -3' miRNA: 3'- caUUGUCGCcCCU---UUGGCAGA-CGGUa -5' |
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| 23550 | 5' | -54.5 | NC_005260.1 | + | 136469 | 0.68 | 0.914098 |
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Target: 5'- -cAGCAGCGuguGGGAuagcACCGUC-GCCAa -3' miRNA: 3'- caUUGUCGC---CCCUu---UGGCAGaCGGUa -5' |
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| 23550 | 5' | -54.5 | NC_005260.1 | + | 82269 | 0.69 | 0.888843 |
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Target: 5'- -gAAUAGCGGGu-GAUUGUCUGCUAUc -3' miRNA: 3'- caUUGUCGCCCcuUUGGCAGACGGUA- -5' |
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| 23550 | 5' | -54.5 | NC_005260.1 | + | 145907 | 0.69 | 0.881958 |
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Target: 5'- -aAACGGCGGGGAAACUc---GCCGUu -3' miRNA: 3'- caUUGUCGCCCCUUUGGcagaCGGUA- -5' |
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| 23550 | 5' | -54.5 | NC_005260.1 | + | 203974 | 1.06 | 0.008298 |
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Target: 5'- gGUAACAGCGGGGAAACCGUCUGCCAUa -3' miRNA: 3'- -CAUUGUCGCCCCUUUGGCAGACGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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