Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23555 | 3' | -48.9 | NC_005260.1 | + | 80581 | 0.66 | 0.999771 |
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Target: 5'- aCCauAGUUGCauUACGACCggGGAUAACu -3' miRNA: 3'- -GGcuUCAACG--GUGCUGGgaCUUAUUG- -5' |
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| 23555 | 3' | -48.9 | NC_005260.1 | + | 49147 | 0.67 | 0.999711 |
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Target: 5'- -aGGAGUUcGCCGCGAUUgaGAuugAUAACa -3' miRNA: 3'- ggCUUCAA-CGGUGCUGGgaCU---UAUUG- -5' |
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| 23555 | 3' | -48.9 | NC_005260.1 | + | 159639 | 0.67 | 0.999711 |
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Target: 5'- cUCGuaacGUUGCCGCuGCUCUGggUcACa -3' miRNA: 3'- -GGCuu--CAACGGUGcUGGGACuuAuUG- -5' |
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| 23555 | 3' | -48.9 | NC_005260.1 | + | 21189 | 0.67 | 0.999637 |
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Target: 5'- -aGAAGUUGCUAguGCCaCUGAAUAc- -3' miRNA: 3'- ggCUUCAACGGUgcUGG-GACUUAUug -5' |
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| 23555 | 3' | -48.9 | NC_005260.1 | + | 155717 | 0.67 | 0.999485 |
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Target: 5'- aCCGAAGUuuccacgcUGCCugGugCgCaauccaugggugaaaUGGAUGACg -3' miRNA: 3'- -GGCUUCA--------ACGGugCugG-G---------------ACUUAUUG- -5' |
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| 23555 | 3' | -48.9 | NC_005260.1 | + | 102176 | 0.67 | 0.999439 |
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Target: 5'- aCGAAGUUgagacuuccgGCCugGuauucaaagauACCCUGAGUGu- -3' miRNA: 3'- gGCUUCAA----------CGGugC-----------UGGGACUUAUug -5' |
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| 23555 | 3' | -48.9 | NC_005260.1 | + | 232807 | 0.71 | 0.990228 |
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Target: 5'- gCGAgacAGUacGCCACGACCCaGAGUGc- -3' miRNA: 3'- gGCU---UCAa-CGGUGCUGGGaCUUAUug -5' |
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| 23555 | 3' | -48.9 | NC_005260.1 | + | 229488 | 1.14 | 0.01112 |
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Target: 5'- aCCGAAGUUGCCACGACCCUGAAUAACa -3' miRNA: 3'- -GGCUUCAACGGUGCUGGGACUUAUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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