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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23556 | 3' | -49.6 | NC_005260.1 | + | 101021 | 0.66 | 0.998573 |
Target: 5'- -cUGGuaucuGCGAuugugguagaaaaUCCUGUAUGGUAGCUc -3' miRNA: 3'- aaACCu----CGCU-------------AGGGUAUACUAUCGAu -5' |
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23556 | 3' | -49.6 | NC_005260.1 | + | 99582 | 0.66 | 0.998309 |
Target: 5'- --aGGAGCGcGUCCCAgAUGAUucaccauGCUu -3' miRNA: 3'- aaaCCUCGC-UAGGGUaUACUAu------CGAu -5' |
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23556 | 3' | -49.6 | NC_005260.1 | + | 109493 | 0.67 | 0.995294 |
Target: 5'- -aUGGAGCaaagguguUCCCAUuacggauuuUGAUAGCUGg -3' miRNA: 3'- aaACCUCGcu------AGGGUAu--------ACUAUCGAU- -5' |
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23556 | 3' | -49.6 | NC_005260.1 | + | 230916 | 0.8 | 0.585376 |
Target: 5'- gUUGGAGCGAUUCUGUGUGAuaUAGCa- -3' miRNA: 3'- aAACCUCGCUAGGGUAUACU--AUCGau -5' |
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23556 | 3' | -49.6 | NC_005260.1 | + | 230619 | 1.05 | 0.023481 |
Target: 5'- aUUUGGAGCGAUCCCAUAUGAUAGCUAu -3' miRNA: 3'- -AAACCUCGCUAGGGUAUACUAUCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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