miRNA: 3'- uaaCGGUaCGGuaugUGCCGGCAGaa -5'
miRNA: 3'- uaaCGGu---ACG-GUAuGUGCCGGCAGAa -5'
miRNA: 3'- uaaCGGU-------ACG--GUAUGUGCCGGCAGaa -5'
miRNA: 3'- uaaCGGUACGGUau------GUGC---CGGCAGaa -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23562 | 3' | -54.8 | NC_005261.1 | + | 12520 | 0.66 | 0.888826 |
|
Target: 5'- --aGCCGaGCCcgcgGCGGCCGUCg- -3' miRNA: 3'- uaaCGGUaCGGuaugUGCCGGCAGaa -5' |
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| 23562 | 3' | -54.8 | NC_005261.1 | + | 8823 | 0.67 | 0.842128 |
|
Target: 5'- --cGCUuuauUGCgCGU-CGCGGCCGUCUc -3' miRNA: 3'- uaaCGGu---ACG-GUAuGUGCCGGCAGAa -5' |
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| 23562 | 3' | -54.8 | NC_005261.1 | + | 6279 | 0.7 | 0.664569 |
|
Target: 5'- --cGCCAaacucauUGUaaUAUGCGCGGCCGUCg- -3' miRNA: 3'- uaaCGGU-------ACG--GUAUGUGCCGGCAGaa -5' |
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| 23562 | 3' | -54.8 | NC_005261.1 | + | 3474 | 0.66 | 0.881624 |
|
Target: 5'- --cGCCAUGCCGccgcggguCGCGgguGCCGUCg- -3' miRNA: 3'- uaaCGGUACGGUau------GUGC---CGGCAGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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