Results 141 - 147 of 147 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23562 | 5' | -49.5 | NC_005261.1 | + | 7195 | 0.66 | 0.997949 |
|
Target: 5'- aGGggGGggUGCGCGUGCguGGCaGCg-- -3' miRNA: 3'- -CCuuUUaaAUGUGCACG--CCGaCGggc -5' |
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| 23562 | 5' | -49.5 | NC_005261.1 | + | 6339 | 0.66 | 0.997417 |
|
Target: 5'- cGGGugcgcgccguCGCGUGCGGCggcgGCCg- -3' miRNA: 3'- -CCUuuuaaau---GUGCACGCCGa---CGGgc -5' |
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| 23562 | 5' | -49.5 | NC_005261.1 | + | 6133 | 0.68 | 0.985526 |
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Target: 5'- --------aGCGCGUcGCGGCcGCCCu -3' miRNA: 3'- ccuuuuaaaUGUGCA-CGCCGaCGGGc -5' |
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| 23562 | 5' | -49.5 | NC_005261.1 | + | 4032 | 0.68 | 0.983651 |
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Target: 5'- ---------cCACGUGCGGCagcGCCCa -3' miRNA: 3'- ccuuuuaaauGUGCACGCCGa--CGGGc -5' |
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| 23562 | 5' | -49.5 | NC_005261.1 | + | 2808 | 0.67 | 0.994457 |
|
Target: 5'- cGGGAGGg--GC-CGgGCGGCcccaGCCCGa -3' miRNA: 3'- -CCUUUUaaaUGuGCaCGCCGa---CGGGC- -5' |
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| 23562 | 5' | -49.5 | NC_005261.1 | + | 1963 | 0.77 | 0.679926 |
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Target: 5'- -------gUACACGUGCGcCUGCCCGa -3' miRNA: 3'- ccuuuuaaAUGUGCACGCcGACGGGC- -5' |
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| 23562 | 5' | -49.5 | NC_005261.1 | + | 433 | 0.72 | 0.904542 |
|
Target: 5'- aGGGGcggcgccgGCGCGgGCGGCgggGCCCGg -3' miRNA: 3'- -CCUUuuaaa---UGUGCaCGCCGa--CGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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