Results 141 - 147 of 147 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23562 | 5' | -49.5 | NC_005261.1 | + | 67999 | 0.67 | 0.992452 |
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Target: 5'- aGGucGAGUUUGCGC--GCGGCgccaacuccagcaUGCCCGu -3' miRNA: 3'- -CCu-UUUAAAUGUGcaCGCCG-------------ACGGGC- -5' |
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| 23562 | 5' | -49.5 | NC_005261.1 | + | 22361 | 0.67 | 0.992559 |
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Target: 5'- aGGGAGAUUgccggGCGCGccucgGCGGggGCCgCGc -3' miRNA: 3'- -CCUUUUAAa----UGUGCa----CGCCgaCGG-GC- -5' |
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| 23562 | 5' | -49.5 | NC_005261.1 | + | 135946 | 0.67 | 0.992559 |
|
Target: 5'- nGGGGAG---GCGCuG-GCGGCgccGCCCGg -3' miRNA: 3'- -CCUUUUaaaUGUG-CaCGCCGa--CGGGC- -5' |
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| 23562 | 5' | -49.5 | NC_005261.1 | + | 137424 | 0.67 | 0.992559 |
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Target: 5'- gGGAAGGggcGCGCGUgggggGCGGCgGCgCGg -3' miRNA: 3'- -CCUUUUaaaUGUGCA-----CGCCGaCGgGC- -5' |
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| 23562 | 5' | -49.5 | NC_005261.1 | + | 134842 | 0.67 | 0.992559 |
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Target: 5'- cGGAAGugccgGCGCGgcugGCGGCcgcGCUCGc -3' miRNA: 3'- -CCUUUuaaa-UGUGCa---CGCCGa--CGGGC- -5' |
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| 23562 | 5' | -49.5 | NC_005261.1 | + | 32306 | 0.67 | 0.993469 |
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Target: 5'- cGGAGGgcgcggcGUggGCGCGcGCGaGCgGCCCGc -3' miRNA: 3'- -CCUUU-------UAaaUGUGCaCGC-CGaCGGGC- -5' |
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| 23562 | 5' | -49.5 | NC_005261.1 | + | 30214 | 0.66 | 0.996554 |
|
Target: 5'- --------cGgACGUGCugccgcugcuggGGCUGCCCGa -3' miRNA: 3'- ccuuuuaaaUgUGCACG------------CCGACGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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