Results 101 - 107 of 107 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23572 | 3' | -53.9 | NC_005261.1 | + | 11705 | 0.66 | 0.953316 |
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Target: 5'- cCGAgGCGcgGCgGGCGCGGGcGcccaggcagaccGCCUGCg -3' miRNA: 3'- -GCU-CGCa-CGaCUGCGUCCaU------------UGGAUG- -5' |
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| 23572 | 3' | -53.9 | NC_005261.1 | + | 55780 | 0.66 | 0.953316 |
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Target: 5'- gGGGCGgcaacgGC-GGCGCGGGgccGGCCggggGCg -3' miRNA: 3'- gCUCGCa-----CGaCUGCGUCCa--UUGGa---UG- -5' |
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| 23572 | 3' | -53.9 | NC_005261.1 | + | 105565 | 0.66 | 0.953316 |
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Target: 5'- gCGGGCGgcgGCguuagcGGCGCGGGggGCUgGCc -3' miRNA: 3'- -GCUCGCa--CGa-----CUGCGUCCauUGGaUG- -5' |
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| 23572 | 3' | -53.9 | NC_005261.1 | + | 110901 | 0.66 | 0.953316 |
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Target: 5'- uGGGCGacUGC-GACGCGacGGUGuuCCUGCc -3' miRNA: 3'- gCUCGC--ACGaCUGCGU--CCAUu-GGAUG- -5' |
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| 23572 | 3' | -53.9 | NC_005261.1 | + | 1047 | 0.66 | 0.953316 |
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Target: 5'- gCGGGCGgcgGCguuagcGGCGCGGGggGCUgGCc -3' miRNA: 3'- -GCUCGCa--CGa-----CUGCGUCCauUGGaUG- -5' |
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| 23572 | 3' | -53.9 | NC_005261.1 | + | 124181 | 0.66 | 0.953316 |
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Target: 5'- aGAGCGUcGCgugggggGACGCGuccGGUGGCgugaaCUGCg -3' miRNA: 3'- gCUCGCA-CGa------CUGCGU---CCAUUG-----GAUG- -5' |
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| 23572 | 3' | -53.9 | NC_005261.1 | + | 116483 | 0.66 | 0.953316 |
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Target: 5'- gCGGGCGggGCgGGCGCGcGGUAuaaagagcgccGCCgcgGCg -3' miRNA: 3'- -GCUCGCa-CGaCUGCGU-CCAU-----------UGGa--UG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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