Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23578 | 5' | -56.9 | NC_005261.1 | + | 65858 | 0.66 | 0.853095 |
Target: 5'- gGAGUGGGAGaggUgGUCGGGGgcgagucggcGGUGCAc -3' miRNA: 3'- -CUCGUCCUCa--GgUAGCUCCa---------CCACGU- -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 37938 | 0.66 | 0.83686 |
Target: 5'- cGAGCGccGGAGcCgCGUCGAGcG-GGUGCu -3' miRNA: 3'- -CUCGU--CCUCaG-GUAGCUC-CaCCACGu -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 72077 | 0.66 | 0.828454 |
Target: 5'- --cCAGGucguAGUCCA-CGAGGUGGcGCGu -3' miRNA: 3'- cucGUCC----UCAGGUaGCUCCACCaCGU- -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 100453 | 0.67 | 0.783859 |
Target: 5'- -cGCgGGGAGUCCGUUGGGGcgccggcgcuUGGgcgGCGc -3' miRNA: 3'- cuCG-UCCUCAGGUAGCUCC----------ACCa--CGU- -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 104240 | 0.67 | 0.764985 |
Target: 5'- cAGCAGGAGcCCAgcucCGGGGcGGcgGCGg -3' miRNA: 3'- cUCGUCCUCaGGUa---GCUCCaCCa-CGU- -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 117421 | 0.68 | 0.735795 |
Target: 5'- gGAGCuGGAGUCCcccUCGGGGUuccGGcgGCu -3' miRNA: 3'- -CUCGuCCUCAGGu--AGCUCCA---CCa-CGu -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 71196 | 0.68 | 0.732826 |
Target: 5'- cGGGCGGGcuggucuccggccaGGUCCugucaaacAUCGAGGUGGccUGCc -3' miRNA: 3'- -CUCGUCC--------------UCAGG--------UAGCUCCACC--ACGu -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 6775 | 0.68 | 0.725869 |
Target: 5'- aGGCGGGAGgcgCCcgCGAGGcgcGGcGCAa -3' miRNA: 3'- cUCGUCCUCa--GGuaGCUCCa--CCaCGU- -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 121900 | 0.68 | 0.71586 |
Target: 5'- cGGGCAGGAGaCCGgcggCGAGG-GGgaggGCc -3' miRNA: 3'- -CUCGUCCUCaGGUa---GCUCCaCCa---CGu -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 66463 | 0.71 | 0.561985 |
Target: 5'- cGAGCGGGccgcGGUCCGgcUgGAGGUGGgGCu -3' miRNA: 3'- -CUCGUCC----UCAGGU--AgCUCCACCaCGu -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 136777 | 0.71 | 0.541772 |
Target: 5'- -uGCAGcGAGUCgG-CGAGGcGGUGCAg -3' miRNA: 3'- cuCGUC-CUCAGgUaGCUCCaCCACGU- -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 34397 | 0.75 | 0.359089 |
Target: 5'- -cGCAGGAGUUUAUCGAccgGGUGGcGCGc -3' miRNA: 3'- cuCGUCCUCAGGUAGCU---CCACCaCGU- -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 91985 | 0.77 | 0.27861 |
Target: 5'- cAGCAGGuGGUCCAgcaGGcGGUGGUGCAg -3' miRNA: 3'- cUCGUCC-UCAGGUag-CU-CCACCACGU- -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 128066 | 0.8 | 0.169865 |
Target: 5'- cGGCAGGAG-CCGUCGcAGGUGGgGCAg -3' miRNA: 3'- cUCGUCCUCaGGUAGC-UCCACCaCGU- -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 23549 | 0.8 | 0.169865 |
Target: 5'- cGGCAGGAG-CCGUCGcAGGUGGgGCAg -3' miRNA: 3'- cUCGUCCUCaGGUAGC-UCCACCaCGU- -5' |
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23578 | 5' | -56.9 | NC_005261.1 | + | 114204 | 1.08 | 0.002133 |
Target: 5'- cGAGCAGGAGUCCAUCGAGGUGGUGCAc -3' miRNA: 3'- -CUCGUCCUCAGGUAGCUCCACCACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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