Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2358 | 3' | -52.7 | NC_001416.1 | + | 3377 | 0.69 | 0.644601 |
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Target: 5'- -aUACCGGcGACAGCCGGAacugccgUGCcGGUg -3' miRNA: 3'- ucGUGGCUaUUGUUGGCCU-------ACGaCCA- -5' |
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| 2358 | 3' | -52.7 | NC_001416.1 | + | 5267 | 0.66 | 0.767598 |
|
Target: 5'- cAGCACCGAUGcgaucaccguCAugCGuGAUGCacUGGa -3' miRNA: 3'- -UCGUGGCUAUu---------GUugGC-CUACG--ACCa -5' |
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| 2358 | 3' | -52.7 | NC_001416.1 | + | 5858 | 0.77 | 0.24863 |
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Target: 5'- cGGUugCGGUAugAGCCGGGUcacuguugcccugcgGCUGGUa -3' miRNA: 3'- -UCGugGCUAUugUUGGCCUA---------------CGACCA- -5' |
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| 2358 | 3' | -52.7 | NC_001416.1 | + | 7762 | 0.69 | 0.611549 |
|
Target: 5'- cGCACCuGAcuGCucACCGGAUGUaaUGGUg -3' miRNA: 3'- uCGUGG-CUauUGu-UGGCCUACG--ACCA- -5' |
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| 2358 | 3' | -52.7 | NC_001416.1 | + | 8755 | 0.66 | 0.778032 |
|
Target: 5'- aGGUGCCGGauuCAgaGCUGGAUGCgUGGa -3' miRNA: 3'- -UCGUGGCUauuGU--UGGCCUACG-ACCa -5' |
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| 2358 | 3' | -52.7 | NC_001416.1 | + | 10911 | 0.7 | 0.566272 |
|
Target: 5'- cGCuggCGGUGGCGACCGGugcGCUGGc -3' miRNA: 3'- uCGug-GCUAUUGUUGGCCua-CGACCa -5' |
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| 2358 | 3' | -52.7 | NC_001416.1 | + | 15238 | 0.69 | 0.634342 |
|
Target: 5'- uGGCACCGAgagaaAACaGGAUGCcGGUc -3' miRNA: 3'- -UCGUGGCUauug-UUGgCCUACGaCCA- -5' |
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| 2358 | 3' | -52.7 | NC_001416.1 | + | 15735 | 0.68 | 0.668485 |
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Target: 5'- -cCACCG-UGAC-ACCGGAuaUGUUGGUa -3' miRNA: 3'- ucGUGGCuAUUGuUGGCCU--ACGACCA- -5' |
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| 2358 | 3' | -52.7 | NC_001416.1 | + | 16955 | 0.67 | 0.735449 |
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Target: 5'- cAGCACCGGUGGCGuguGCUGGcgGUg--- -3' miRNA: 3'- -UCGUGGCUAUUGU---UGGCCuaCGacca -5' |
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| 2358 | 3' | -52.7 | NC_001416.1 | + | 21883 | 1.08 | 0.001546 |
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Target: 5'- gAGCACCGAUAACAACCGGAUGCUGGUg -3' miRNA: 3'- -UCGUGGCUAUUGUUGGCCUACGACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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