Results 121 - 126 of 126 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23588 | 5' | -54.9 | NC_005261.1 | + | 32392 | 0.67 | 0.890003 |
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Target: 5'- cGCGGCG-GCGUcccGgagcGCGUGCcgcCGCCGGc -3' miRNA: 3'- -CGCCGCaCGUA---Ca---UGUACGa--GCGGUC- -5' |
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| 23588 | 5' | -54.9 | NC_005261.1 | + | 38494 | 0.67 | 0.894762 |
|
Target: 5'- gGCGGCGUcgacuggaGCGUcGUAUAaggcgcgcgcuggcUGCgcgCGCCGGg -3' miRNA: 3'- -CGCCGCA--------CGUA-CAUGU--------------ACGa--GCGGUC- -5' |
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| 23588 | 5' | -54.9 | NC_005261.1 | + | 39118 | 0.67 | 0.8961 |
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Target: 5'- cCGGUGUaCGUGUACGaguuuucUGC-CGCCGGc -3' miRNA: 3'- cGCCGCAcGUACAUGU-------ACGaGCGGUC- -5' |
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| 23588 | 5' | -54.9 | NC_005261.1 | + | 88455 | 0.67 | 0.8961 |
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Target: 5'- cGCGccGCGUgaGCGUGUGggcgggcCGUGCcgCGCCGGa -3' miRNA: 3'- -CGC--CGCA--CGUACAU-------GUACGa-GCGGUC- -5' |
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| 23588 | 5' | -54.9 | NC_005261.1 | + | 15065 | 0.67 | 0.896766 |
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Target: 5'- cGCGGCGggGUcgGggguCAgGCUCGCCc- -3' miRNA: 3'- -CGCCGCa-CGuaCau--GUaCGAGCGGuc -5' |
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| 23588 | 5' | -54.9 | NC_005261.1 | + | 72468 | 0.66 | 0.92694 |
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Target: 5'- cGCGGCGaGCGcGaGCGcgGCcagCGCCAGg -3' miRNA: 3'- -CGCCGCaCGUaCaUGUa-CGa--GCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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