Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2359 | 3' | -57.9 | NC_001416.1 | + | 5420 | 0.66 | 0.488945 |
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Target: 5'- cGAGaaCGCCAGCgCGG-CGCAGCcggACGUg -3' miRNA: 3'- -UUCaaGUGGUCG-GUCgGCGUCG---UGCA- -5' |
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| 2359 | 3' | -57.9 | NC_001416.1 | + | 4741 | 0.67 | 0.438217 |
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Target: 5'- cGGcUgACCAGCguGCCggacaccgGCAGCACGg -3' miRNA: 3'- uUCaAgUGGUCGguCGG--------CGUCGUGCa -5' |
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| 2359 | 3' | -57.9 | NC_001416.1 | + | 2446 | 0.67 | 0.438217 |
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Target: 5'- --cUUCAgCAGUCAGCUGCuGCGCu- -3' miRNA: 3'- uucAAGUgGUCGGUCGGCGuCGUGca -5' |
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| 2359 | 3' | -57.9 | NC_001416.1 | + | 11138 | 0.67 | 0.438217 |
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Target: 5'- -cGUcCAUCAGCCAGagUGUGGCGCGUu -3' miRNA: 3'- uuCAaGUGGUCGGUCg-GCGUCGUGCA- -5' |
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| 2359 | 3' | -57.9 | NC_001416.1 | + | 5310 | 0.74 | 0.140495 |
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Target: 5'- cAAGUUCAUCAGCCAGUC-CGGCAUc- -3' miRNA: 3'- -UUCAAGUGGUCGGUCGGcGUCGUGca -5' |
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| 2359 | 3' | -57.9 | NC_001416.1 | + | 24235 | 1.04 | 0.000741 |
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Target: 5'- gAAGUUCACCAGCCAGCCGCAGCACGUu -3' miRNA: 3'- -UUCAAGUGGUCGGUCGGCGUCGUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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