Results 1 - 20 of 82 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23593 | 3' | -59.8 | NC_005261.1 | + | 82255 | 0.66 | 0.706652 |
Target: 5'- aCGCG-CAGGGUCUuCUCCAGCucccGCc- -3' miRNA: 3'- -GCGUgGUCCCAGAuGGGGUCGu---CGua -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 103281 | 0.66 | 0.700648 |
Target: 5'- gCGCGCCGGGuGcCgcgcgcaGCCCCgcgaagcccugcagcGGCAGCAg -3' miRNA: 3'- -GCGUGGUCC-CaGa------UGGGG---------------UCGUCGUa -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 36160 | 0.66 | 0.696633 |
Target: 5'- gCGCAUgcgCGGGGcuuuuaUGCCgCCGGCAGCGc -3' miRNA: 3'- -GCGUG---GUCCCag----AUGG-GGUCGUCGUa -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 110448 | 0.66 | 0.686561 |
Target: 5'- cCGCACCGGGcGUUugggUGCCUgGGCGGg-- -3' miRNA: 3'- -GCGUGGUCC-CAG----AUGGGgUCGUCgua -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 85994 | 0.66 | 0.685551 |
Target: 5'- uGCGCCgccgacgGGGcGUCgUGCgCCAGCAGCc- -3' miRNA: 3'- gCGUGG-------UCC-CAG-AUGgGGUCGUCGua -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 101735 | 0.66 | 0.676443 |
Target: 5'- cCGCgAUCAGGGcCUcgcucggcaGCUCCAGguGCAc -3' miRNA: 3'- -GCG-UGGUCCCaGA---------UGGGGUCguCGUa -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 39173 | 0.66 | 0.676443 |
Target: 5'- aCGCGCUcGGGcccgaCUcgcGCCCCGGCgAGCAg -3' miRNA: 3'- -GCGUGGuCCCa----GA---UGGGGUCG-UCGUa -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 39560 | 0.66 | 0.67543 |
Target: 5'- gCGCGCgCGGcGGUCUGucccccucucccuCCCCAGCgccgAGCGc -3' miRNA: 3'- -GCGUG-GUC-CCAGAU-------------GGGGUCG----UCGUa -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 102775 | 0.66 | 0.66629 |
Target: 5'- gCGCGCCcGaGGcCUcGCCCCGGCcGCGa -3' miRNA: 3'- -GCGUGGuC-CCaGA-UGGGGUCGuCGUa -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 104660 | 0.66 | 0.66629 |
Target: 5'- aCGCGCUggcGGGcGUgUGCUgCGGCAGCGc -3' miRNA: 3'- -GCGUGG---UCC-CAgAUGGgGUCGUCGUa -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 106803 | 0.66 | 0.66629 |
Target: 5'- cCGCACCAGcGcCgagugGCCcgCCAGCAGCGc -3' miRNA: 3'- -GCGUGGUCcCaGa----UGG--GGUCGUCGUa -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 58006 | 0.66 | 0.656109 |
Target: 5'- aGCGCCAGGacguggaagUGCCgCGGCGGCAg -3' miRNA: 3'- gCGUGGUCCcag------AUGGgGUCGUCGUa -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 33065 | 0.67 | 0.645909 |
Target: 5'- gGCGCCGGGGaCgcgGCgCUGGCGGCc- -3' miRNA: 3'- gCGUGGUCCCaGa--UGgGGUCGUCGua -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 31039 | 0.67 | 0.645909 |
Target: 5'- gCGCGCCGccGGGU---CCCCAGCgcGGCGg -3' miRNA: 3'- -GCGUGGU--CCCAgauGGGGUCG--UCGUa -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 15927 | 0.67 | 0.645909 |
Target: 5'- gGgGCCGGGGcCgggGCCCgGGCAGgAg -3' miRNA: 3'- gCgUGGUCCCaGa--UGGGgUCGUCgUa -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 85235 | 0.67 | 0.635698 |
Target: 5'- cCGCGCCGGcaucuGGUUgcGCUgCAGCAGCGUc -3' miRNA: 3'- -GCGUGGUC-----CCAGa-UGGgGUCGUCGUA- -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 14926 | 0.67 | 0.635698 |
Target: 5'- gGCACCAGGaUCgACgCCAGCuGGCGc -3' miRNA: 3'- gCGUGGUCCcAGaUGgGGUCG-UCGUa -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 96630 | 0.67 | 0.635698 |
Target: 5'- aGcCGCCGGGcGaUCUucucgcagGCCuCCAGCAGCAg -3' miRNA: 3'- gC-GUGGUCC-C-AGA--------UGG-GGUCGUCGUa -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 65863 | 0.67 | 0.625484 |
Target: 5'- cCGCGgCGGcuuGUacaGCCCCAGCGGCAUg -3' miRNA: 3'- -GCGUgGUCc--CAga-UGGGGUCGUCGUA- -5' |
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23593 | 3' | -59.8 | NC_005261.1 | + | 52436 | 0.67 | 0.625484 |
Target: 5'- cCGCGuCCAGGGcCacgagguCCCCGGCGcGCGUc -3' miRNA: 3'- -GCGU-GGUCCCaGau-----GGGGUCGU-CGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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