Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23597 | 3' | -52.4 | NC_005261.1 | + | 121109 | 0.71 | 0.816732 |
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Target: 5'- cGUGCGgguccucuagggCgcGGAGGCGcggGCGCGAGCGc -3' miRNA: 3'- -CAUGCa-----------GaaCUUCCGCa--UGCGCUCGC- -5' |
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| 23597 | 3' | -52.4 | NC_005261.1 | + | 122617 | 0.67 | 0.959274 |
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Target: 5'- cGUGCcugugCUUGAAcGGCGaGCGCGAGg- -3' miRNA: 3'- -CAUGca---GAACUU-CCGCaUGCGCUCgc -5' |
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| 23597 | 3' | -52.4 | NC_005261.1 | + | 125401 | 0.69 | 0.921644 |
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Target: 5'- gGUACGUUccGGA-GCG-GCGCGGGCGg -3' miRNA: 3'- -CAUGCAGaaCUUcCGCaUGCGCUCGC- -5' |
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| 23597 | 3' | -52.4 | NC_005261.1 | + | 125800 | 0.66 | 0.969424 |
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Target: 5'- -aACGgggCUUGGgccgGGGCcgcCGCGAGCGg -3' miRNA: 3'- caUGCa--GAACU----UCCGcauGCGCUCGC- -5' |
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| 23597 | 3' | -52.4 | NC_005261.1 | + | 127736 | 0.67 | 0.966266 |
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Target: 5'- -aGCGggagCUUGgcGGCGggaGCGGGCc -3' miRNA: 3'- caUGCa---GAACuuCCGCaugCGCUCGc -5' |
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| 23597 | 3' | -52.4 | NC_005261.1 | + | 134893 | 0.66 | 0.975097 |
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Target: 5'- uGUGCGUUcgguggUGGAGcGgGUGCGCaAGCGc -3' miRNA: 3'- -CAUGCAGa-----ACUUC-CgCAUGCGcUCGC- -5' |
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| 23597 | 3' | -52.4 | NC_005261.1 | + | 137037 | 0.69 | 0.914617 |
|
Target: 5'- -gGCGUCggugGAGGGCGgaccgucggcagGCGCGgaGGCGc -3' miRNA: 3'- caUGCAGaa--CUUCCGCa-----------UGCGC--UCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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