Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23597 | 3' | -52.4 | NC_005261.1 | + | 29340 | 0.68 | 0.932548 |
|
Target: 5'- cGUGCG-CUUc--GGCG-GCGCGGGCGa -3' miRNA: 3'- -CAUGCaGAAcuuCCGCaUGCGCUCGC- -5' |
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| 23597 | 3' | -52.4 | NC_005261.1 | + | 22768 | 0.69 | 0.903401 |
|
Target: 5'- -cGCGUC-UGggGGCGggcccCGCGAGa- -3' miRNA: 3'- caUGCAGaACuuCCGCau---GCGCUCgc -5' |
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| 23597 | 3' | -52.4 | NC_005261.1 | + | 21006 | 0.71 | 0.835847 |
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Target: 5'- -cGCGcCUUGggGGCGUGacacCGAGCc -3' miRNA: 3'- caUGCaGAACuuCCGCAUgc--GCUCGc -5' |
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| 23597 | 3' | -52.4 | NC_005261.1 | + | 19476 | 0.66 | 0.977626 |
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Target: 5'- --cCGUCUUGAcgucccgauGGGCGauccgGCGgGAGUGc -3' miRNA: 3'- cauGCAGAACU---------UCCGCa----UGCgCUCGC- -5' |
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| 23597 | 3' | -52.4 | NC_005261.1 | + | 15706 | 0.66 | 0.979961 |
|
Target: 5'- uUGCGUCggcugggUGGAcGGCGaACGCGA-CGg -3' miRNA: 3'- cAUGCAGa------ACUU-CCGCaUGCGCUcGC- -5' |
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| 23597 | 3' | -52.4 | NC_005261.1 | + | 13670 | 0.67 | 0.959274 |
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Target: 5'- -cGCGgg--GggGGCGcggggcGCGCGGGCGu -3' miRNA: 3'- caUGCagaaCuuCCGCa-----UGCGCUCGC- -5' |
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| 23597 | 3' | -52.4 | NC_005261.1 | + | 3438 | 0.67 | 0.966266 |
|
Target: 5'- -aGCGUCc---AGGCGggcGCGCGGGCc -3' miRNA: 3'- caUGCAGaacuUCCGCa--UGCGCUCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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