Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 236 | 3' | -58.5 | AC_000008.1 | + | 23779 | 0.66 | 0.436711 |
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Target: 5'- ---cGCCGAgGUCgAUGGCCgCGGGc -3' miRNA: 3'- cauuCGGCUgCAGgUGUCGGgGCCUc -5' |
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| 236 | 3' | -58.5 | AC_000008.1 | + | 18870 | 0.66 | 0.427007 |
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Target: 5'- -cGGGCCagGACGccucggaguaCCugAGCCCCGGGc -3' miRNA: 3'- caUUCGG--CUGCa---------GGugUCGGGGCCUc -5' |
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| 236 | 3' | -58.5 | AC_000008.1 | + | 22950 | 0.66 | 0.417432 |
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Target: 5'- -gGAGCCGcACcccCCACAGCCCCu--- -3' miRNA: 3'- caUUCGGC-UGca-GGUGUCGGGGccuc -5' |
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| 236 | 3' | -58.5 | AC_000008.1 | + | 12152 | 0.66 | 0.407988 |
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Target: 5'- -cAAGCCGACGc-----GCCCUGGAGg -3' miRNA: 3'- caUUCGGCUGCagguguCGGGGCCUC- -5' |
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| 236 | 3' | -58.5 | AC_000008.1 | + | 10366 | 0.68 | 0.328484 |
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Target: 5'- -gGGGCCaGCGUaggguggCCGgGGCUCCGGGGg -3' miRNA: 3'- caUUCGGcUGCA-------GGUgUCGGGGCCUC- -5' |
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| 236 | 3' | -58.5 | AC_000008.1 | + | 26881 | 0.68 | 0.321273 |
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Target: 5'- -gGGGgCGugGUCUACAGCCgcguCUGGAa -3' miRNA: 3'- caUUCgGCugCAGGUGUCGG----GGCCUc -5' |
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| 236 | 3' | -58.5 | AC_000008.1 | + | 6553 | 0.69 | 0.283375 |
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Target: 5'- -gGGGUCuGCGUCCACGGuaaagaCCCCGGGc -3' miRNA: 3'- caUUCGGcUGCAGGUGUC------GGGGCCUc -5' |
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| 236 | 3' | -58.5 | AC_000008.1 | + | 25834 | 0.73 | 0.140928 |
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Target: 5'- ---cGCCuGACGaaaagUCCGCGGCUCCGGGGu -3' miRNA: 3'- cauuCGG-CUGC-----AGGUGUCGGGGCCUC- -5' |
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| 236 | 3' | -58.5 | AC_000008.1 | + | 24880 | 0.78 | 0.06311 |
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Target: 5'- aGUGAGCUGAucgugcgcCGUgCGCAGCCCCuGGAGa -3' miRNA: 3'- -CAUUCGGCU--------GCAgGUGUCGGGG-CCUC- -5' |
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| 236 | 3' | -58.5 | AC_000008.1 | + | 10005 | 1.07 | 0.000361 |
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Target: 5'- gGUAAGCCGACGUCCACAGCCCCGGAGu -3' miRNA: 3'- -CAUUCGGCUGCAGGUGUCGGGGCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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