Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2360 | 5' | -46.6 | NC_001416.1 | + | 45707 | 0.66 | 0.986303 |
Target: 5'- gGAAAGAAGCUGG-UAGCG---UCCGGc -3' miRNA: 3'- -UUUUUUUCGGUCuGUCGUcaaAGGCC- -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 43627 | 0.72 | 0.821657 |
Target: 5'- -uGGAAAGCCAGuugaucauCAGCAGguaaUCUGGa -3' miRNA: 3'- uuUUUUUCGGUCu-------GUCGUCaa--AGGCC- -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 42810 | 0.68 | 0.950157 |
Target: 5'- -uGAGGuGCC--GCAGCAGUUgUCCGGu -3' miRNA: 3'- uuUUUUuCGGucUGUCGUCAA-AGGCC- -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 39479 | 0.68 | 0.945131 |
Target: 5'- cGGAGuuuGGCCGGGCucAGCAcGUUaCCGGa -3' miRNA: 3'- -UUUUuu-UCGGUCUG--UCGU-CAAaGGCC- -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 30886 | 1.1 | 0.005059 |
Target: 5'- cAAAAAAAGCCAGACAGCAGUUUCCGGa -3' miRNA: 3'- -UUUUUUUCGGUCUGUCGUCAAAGGCC- -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 18576 | 0.7 | 0.885298 |
Target: 5'- --uGAucGCCAGAUAGUGGUgcUUCCGc -3' miRNA: 3'- uuuUUuuCGGUCUGUCGUCA--AAGGCc -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 12901 | 0.67 | 0.97067 |
Target: 5'- -cAGAcAGCCGGuC-GCAGgcgUCCGGg -3' miRNA: 3'- uuUUUuUCGGUCuGuCGUCaa-AGGCC- -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 9817 | 0.71 | 0.841192 |
Target: 5'- ---cGGAGUCAGACAGCAa---CCGGa -3' miRNA: 3'- uuuuUUUCGGUCUGUCGUcaaaGGCC- -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 9209 | 0.68 | 0.950157 |
Target: 5'- -cGGAGAGCagGGGCAGCAGgcgcugCUGGc -3' miRNA: 3'- uuUUUUUCGg-UCUGUCGUCaaa---GGCC- -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 8693 | 0.68 | 0.96336 |
Target: 5'- -cGAAGAGCUGGACAGCGauacCUGGc -3' miRNA: 3'- uuUUUUUCGGUCUGUCGUcaaaGGCC- -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 6806 | 0.66 | 0.982048 |
Target: 5'- ----cGAGCUggAGACAGCGGUgaaagacCUGGg -3' miRNA: 3'- uuuuuUUCGG--UCUGUCGUCAaa-----GGCC- -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 5867 | 0.66 | 0.988117 |
Target: 5'- ------cGCCGGGC-GCGGUUgcgguaugagCCGGg -3' miRNA: 3'- uuuuuuuCGGUCUGuCGUCAAa---------GGCC- -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 5420 | 0.66 | 0.988117 |
Target: 5'- cGAGAAcGCCAGcGCGGCGcag-CCGGa -3' miRNA: 3'- uUUUUUuCGGUC-UGUCGUcaaaGGCC- -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 4536 | 0.67 | 0.97067 |
Target: 5'- -----cAGCCuGACGgauGCGGUgUCCGGc -3' miRNA: 3'- uuuuuuUCGGuCUGU---CGUCAaAGGCC- -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 3679 | 0.66 | 0.987052 |
Target: 5'- gAGAGuGAGCUGGAUAcGCAGUcagcgauggauuuuaUUCUGGg -3' miRNA: 3'- -UUUUuUUCGGUCUGU-CGUCA---------------AAGGCC- -5' |
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2360 | 5' | -46.6 | NC_001416.1 | + | 591 | 0.66 | 0.982048 |
Target: 5'- ------uGUCGGuGCAGCGGcguUUUCCGGa -3' miRNA: 3'- uuuuuuuCGGUC-UGUCGUC---AAAGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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