Results 81 - 84 of 84 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
23606 | 5' | -56 | NC_005261.1 | + | 136933 | 0.66 | 0.874741 |
Target: 5'- -cGCAUguggugguaCAUgGGCCGcGUCGU-CGGGCa -3' miRNA: 3'- gaCGUA---------GUAgCCGGU-CAGCAgGUCCG- -5' |
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23606 | 5' | -56 | NC_005261.1 | + | 20028 | 0.66 | 0.874741 |
Target: 5'- -cGCgGUCAgcgGGCCGGUucCGUCC-GGCg -3' miRNA: 3'- gaCG-UAGUag-CCGGUCA--GCAGGuCCG- -5' |
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23606 | 5' | -56 | NC_005261.1 | + | 103497 | 0.66 | 0.867345 |
Target: 5'- -aGaCGUCGUCGGCgccgcggacggCGG-CGUCCGcGGCa -3' miRNA: 3'- gaC-GUAGUAGCCG-----------GUCaGCAGGU-CCG- -5' |
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23606 | 5' | -56 | NC_005261.1 | + | 102831 | 0.66 | 0.904607 |
Target: 5'- uCUGCGccUCGcgccgcgccacgugcUgGGCCAGcagcgCGUCCAGGa -3' miRNA: 3'- -GACGU--AGU---------------AgCCGGUCa----GCAGGUCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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