Results 81 - 84 of 84 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
23606 | 5' | -56 | NC_005261.1 | + | 110511 | 0.76 | 0.351452 |
Target: 5'- -aGCGUCA-CGGCC-GUCGUCUgcgGGGCg -3' miRNA: 3'- gaCGUAGUaGCCGGuCAGCAGG---UCCG- -5' |
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23606 | 5' | -56 | NC_005261.1 | + | 104187 | 0.8 | 0.23017 |
Target: 5'- -cGgGUCGUCGGgCGGgacggCGUCCAGGCg -3' miRNA: 3'- gaCgUAGUAGCCgGUCa----GCAGGUCCG- -5' |
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23606 | 5' | -56 | NC_005261.1 | + | 43371 | 0.8 | 0.224589 |
Target: 5'- gCUGCGgcggCAUCGGCCucgcGGUgccggcacCGUCCAGGCg -3' miRNA: 3'- -GACGUa---GUAGCCGG----UCA--------GCAGGUCCG- -5' |
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23606 | 5' | -56 | NC_005261.1 | + | 86748 | 1.12 | 0.001512 |
Target: 5'- cCUGCAUCAUCGGCCAGUCGUCCAGGCa -3' miRNA: 3'- -GACGUAGUAGCCGGUCAGCAGGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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