miRNA: 3'- gaCGUaGUagCCGGUCaGCAGGUCCG- -5'
miRNA: 3'- -GACGuaGua-GCCGGUCagca--GGUCCG- -5'
miRNA: 3'- gaC-GUAGuaGCCGGUCAG------CAGGU-CCG- -5'
miRNA: 3'- gaCGUaGuaGCCGGUCa--GCa--GGUCCG- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23606 | 5' | -56 | NC_005261.1 | + | 3426 | 0.74 | 0.482659 |
|
Target: 5'- -aGCG-CGcgGGCCAG-CGUCCAGGCg -3' miRNA: 3'- gaCGUaGUagCCGGUCaGCAGGUCCG- -5' |
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| 23606 | 5' | -56 | NC_005261.1 | + | 2793 | 0.68 | 0.782742 |
|
Target: 5'- gCUGCcgCcggCGGCCGGgaggggCCGGGCg -3' miRNA: 3'- -GACGuaGua-GCCGGUCagca--GGUCCG- -5' |
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| 23606 | 5' | -56 | NC_005261.1 | + | 1989 | 0.71 | 0.643548 |
|
Target: 5'- -gGcCAUCggCGGCCAGUUcgcgggGUCCAcGGCg -3' miRNA: 3'- gaC-GUAGuaGCCGGUCAG------CAGGU-CCG- -5' |
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| 23606 | 5' | -56 | NC_005261.1 | + | 1179 | 0.7 | 0.714903 |
|
Target: 5'- -cGCG-CcgCGGCCGGggcCGgggCCGGGCg -3' miRNA: 3'- gaCGUaGuaGCCGGUCa--GCa--GGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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