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miRNA: 3'- gUCGUgCCCGaCCUUGa------CGC-GCGCUu -5'
miRNA: 3'- -GUCGUGC-CCGacCU----UGaCGCGCGCUU- -5'
miRNA: 3'- guCGuGCCCGaCC-UUGa---CGCGCGCUu -5'
Results 121 - 123 of 123 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 23609 | 3' | -59.7 | NC_005261.1 | + | 97744 | 0.66 | 0.67458 |
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Target: 5'- uGGCAgGGGC-GGGGCgggcucgGCGgCGCGGc -3' miRNA: 3'- gUCGUgCCCGaCCUUGa------CGC-GCGCUu -5' |
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| 23609 | 3' | -59.7 | NC_005261.1 | + | 44246 | 0.66 | 0.67458 |
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Target: 5'- cCAGCGCGcGGCc-GAcgccGCgGCGCGCGGGg -3' miRNA: 3'- -GUCGUGC-CCGacCU----UGaCGCGCGCUU- -5' |
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| 23609 | 3' | -59.7 | NC_005261.1 | + | 59724 | 0.66 | 0.704836 |
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Target: 5'- -cGC-CGGGC-GGcAGCggcgGCGCGCGGc -3' miRNA: 3'- guCGuGCCCGaCC-UUGa---CGCGCGCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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