miRNA: 3'- -GUCGUGCCCGa-----CCU------UGACGCG-CGCUU- -5'
miRNA: 3'- gUCGUGCCCGa------------CCUUGA----CG-CGCGCUu -5'
miRNA: 3'- -GUCGUGccCG-ACCUUGaCGCGCGCuu -5'
Results 121 - 123 of 123 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23609 | 3' | -59.7 | NC_005261.1 | + | 135452 | 0.78 | 0.14089 |
|
Target: 5'- gCGGCGCGGGCgcugcgGGAccucguACUGCGCgGCGAGa -3' miRNA: 3'- -GUCGUGCCCGa-----CCU------UGACGCG-CGCUU- -5' |
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| 23609 | 3' | -59.7 | NC_005261.1 | + | 135659 | 0.69 | 0.528791 |
|
Target: 5'- gGGCGCGGGCggcccucgcucgaGGAGCUcugcGCcGCGCGGc -3' miRNA: 3'- gUCGUGCCCGa------------CCUUGA----CG-CGCGCUu -5' |
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| 23609 | 3' | -59.7 | NC_005261.1 | + | 136600 | 0.67 | 0.654221 |
|
Target: 5'- cCGGCGCa-GCgUGGAGCgGCGCGCGc- -3' miRNA: 3'- -GUCGUGccCG-ACCUUGaCGCGCGCuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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