Results 121 - 123 of 123 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23609 | 3' | -59.7 | NC_005261.1 | + | 2546 | 0.66 | 0.704836 |
Target: 5'- cCAGCgccGCGGcGCUGGGcGCggGCGUGUGGu -3' miRNA: 3'- -GUCG---UGCC-CGACCU-UGa-CGCGCGCUu -5' |
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23609 | 3' | -59.7 | NC_005261.1 | + | 1918 | 0.68 | 0.582829 |
Target: 5'- cCAGCGCGcGGCgc--ACUGCGcCGCGGg -3' miRNA: 3'- -GUCGUGC-CCGaccuUGACGC-GCGCUu -5' |
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23609 | 3' | -59.7 | NC_005261.1 | + | 1725 | 0.69 | 0.532737 |
Target: 5'- gCGGCACGGGCacc-GCgGUGCGCGGg -3' miRNA: 3'- -GUCGUGCCCGaccuUGaCGCGCGCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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