miRNA: 3'- gUUACGa-CUCUau--CGACUUCuCUACC- -5'
miRNA: 3'- gUUACGACu-------------CUAUCgACUUCUCUACC- -5'
miRNA: 3'- -GUUAcGACU---CUAUCGACu---UCUCUACC- -5'
miRNA: 3'- gUUACG-ACU-CUAUC-GACu-----UCUCUACC- -5'
miRNA: 3'- -GUUACGACUCUAUCGACUUCUCUACC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2361 | 5' | -46.8 | NC_001416.1 | + | 490 | 0.7 | 0.888486 |
|
Target: 5'- gAAUGCcaGAGAcuccGCUGAAGuGGUGGa -3' miRNA: 3'- gUUACGa-CUCUau--CGACUUCuCUACC- -5' |
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| 2361 | 5' | -46.8 | NC_001416.1 | + | 19932 | 0.7 | 0.891625 |
|
Target: 5'- aGGUGCUGcgucgucuugaacuGAUGG-UGAAGAGGUGGc -3' miRNA: 3'- gUUACGACu-------------CUAUCgACUUCUCUACC- -5' |
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| 2361 | 5' | -46.8 | NC_001416.1 | + | 43930 | 0.69 | 0.936128 |
|
Target: 5'- cCGGUaCUGAuguGAUGGCUGcuauGGGGAUGGc -3' miRNA: 3'- -GUUAcGACU---CUAUCGACu---UCUCUACC- -5' |
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| 2361 | 5' | -46.8 | NC_001416.1 | + | 13050 | 0.68 | 0.951739 |
|
Target: 5'- aGAUGCgUGAuGGUGGcCUGuucuccGGAGGUGGa -3' miRNA: 3'- gUUACG-ACU-CUAUC-GACu-----UCUCUACC- -5' |
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| 2361 | 5' | -46.8 | NC_001416.1 | + | 36387 | 1.11 | 0.004402 |
|
Target: 5'- cCAAUGCUGAGAUAGCUGAAGAGAUGGc -3' miRNA: 3'- -GUUACGACUCUAUCGACUUCUCUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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