Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23614 | 5' | -55.5 | NC_005261.1 | + | 133353 | 0.73 | 0.563931 |
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Target: 5'- ---cGGCGCUgC-CGCGCAGGG-CUUCg -3' miRNA: 3'- gcauCUGCGA-GuGCGCGUCCCaGAAG- -5' |
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| 23614 | 5' | -55.5 | NC_005261.1 | + | 104171 | 0.74 | 0.494318 |
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Target: 5'- cCGcGGGCGC-CGCGCGC-GGGUCgUCg -3' miRNA: 3'- -GCaUCUGCGaGUGCGCGuCCCAGaAG- -5' |
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| 23614 | 5' | -55.5 | NC_005261.1 | + | 73729 | 0.75 | 0.419959 |
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Target: 5'- gCGUGcGCGCUUGCGCGCGGGGg--UCc -3' miRNA: 3'- -GCAUcUGCGAGUGCGCGUCCCagaAG- -5' |
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| 23614 | 5' | -55.5 | NC_005261.1 | + | 67058 | 0.77 | 0.360751 |
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Target: 5'- uGUAGGCGggCGCGCGCGGGGgCUcgcUCg -3' miRNA: 3'- gCAUCUGCgaGUGCGCGUCCCaGA---AG- -5' |
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| 23614 | 5' | -55.5 | NC_005261.1 | + | 47824 | 0.77 | 0.352781 |
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Target: 5'- --aGGGCGCUCACGUaCAGGGcCUUCc -3' miRNA: 3'- gcaUCUGCGAGUGCGcGUCCCaGAAG- -5' |
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| 23614 | 5' | -55.5 | NC_005261.1 | + | 57116 | 0.77 | 0.337219 |
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Target: 5'- --aGGACGCgCACGCGCAGGGUg--- -3' miRNA: 3'- gcaUCUGCGaGUGCGCGUCCCAgaag -5' |
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| 23614 | 5' | -55.5 | NC_005261.1 | + | 82243 | 1.1 | 0.002621 |
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Target: 5'- cCGUAGACGCUCACGCGCAGGGUCUUCu -3' miRNA: 3'- -GCAUCUGCGAGUGCGCGUCCCAGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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