Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23614 | 5' | -55.5 | NC_005261.1 | + | 105551 | 0.66 | 0.897799 |
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Target: 5'- -cUGGcCGCaCGCGCGCAGGGcCa-- -3' miRNA: 3'- gcAUCuGCGaGUGCGCGUCCCaGaag -5' |
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| 23614 | 5' | -55.5 | NC_005261.1 | + | 120088 | 0.69 | 0.757083 |
|
Target: 5'- cCGUGGAgGCUCggcacGCGCGCGacGUCUUUa -3' miRNA: 3'- -GCAUCUgCGAG-----UGCGCGUccCAGAAG- -5' |
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| 23614 | 5' | -55.5 | NC_005261.1 | + | 120952 | 0.71 | 0.646194 |
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Target: 5'- gGUGGAgGCUCGCGCcccGCGGGG-Cg-- -3' miRNA: 3'- gCAUCUgCGAGUGCG---CGUCCCaGaag -5' |
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| 23614 | 5' | -55.5 | NC_005261.1 | + | 125517 | 0.66 | 0.897143 |
|
Target: 5'- aGUGGcuuuuauACGCUCGCGCcgcccGCAGGG-CggCg -3' miRNA: 3'- gCAUC-------UGCGAGUGCG-----CGUCCCaGaaG- -5' |
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| 23614 | 5' | -55.5 | NC_005261.1 | + | 125940 | 0.68 | 0.821364 |
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Target: 5'- gGUAGcCGCUg--GCGCGGGGUCg-- -3' miRNA: 3'- gCAUCuGCGAgugCGCGUCCCAGaag -5' |
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| 23614 | 5' | -55.5 | NC_005261.1 | + | 128101 | 0.66 | 0.904226 |
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Target: 5'- --cGGGCGagcggCGCgGCGCGGGGUCg-- -3' miRNA: 3'- gcaUCUGCga---GUG-CGCGUCCCAGaag -5' |
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| 23614 | 5' | -55.5 | NC_005261.1 | + | 133353 | 0.73 | 0.563931 |
|
Target: 5'- ---cGGCGCUgC-CGCGCAGGG-CUUCg -3' miRNA: 3'- gcauCUGCGA-GuGCGCGUCCCaGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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