Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23614 | 5' | -55.5 | NC_005261.1 | + | 47824 | 0.77 | 0.352781 |
Target: 5'- --aGGGCGCUCACGUaCAGGGcCUUCc -3' miRNA: 3'- gcaUCUGCGAGUGCGcGUCCCaGAAG- -5' |
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23614 | 5' | -55.5 | NC_005261.1 | + | 40354 | 0.67 | 0.877119 |
Target: 5'- uCGUGGG-GCUCGCGCaGC-GGGUUgaggUUCa -3' miRNA: 3'- -GCAUCUgCGAGUGCG-CGuCCCAG----AAG- -5' |
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23614 | 5' | -55.5 | NC_005261.1 | + | 40295 | 0.68 | 0.821364 |
Target: 5'- uGUGGACGUUCucguCGCGgAGGGcggCUa- -3' miRNA: 3'- gCAUCUGCGAGu---GCGCgUCCCa--GAag -5' |
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23614 | 5' | -55.5 | NC_005261.1 | + | 35460 | 0.7 | 0.717675 |
Target: 5'- -cUAGGCGCggcgCGCGCGCgucccGGGGUCc-- -3' miRNA: 3'- gcAUCUGCGa---GUGCGCG-----UCCCAGaag -5' |
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23614 | 5' | -55.5 | NC_005261.1 | + | 31590 | 0.7 | 0.707612 |
Target: 5'- aCGUgAGcGCGCUCgGCGCGCAGGGcgugCUg- -3' miRNA: 3'- -GCA-UC-UGCGAG-UGCGCGUCCCa---GAag -5' |
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23614 | 5' | -55.5 | NC_005261.1 | + | 17476 | 0.68 | 0.803744 |
Target: 5'- --cAGACGCggGCGCGCcggGGGGUCc-- -3' miRNA: 3'- gcaUCUGCGagUGCGCG---UCCCAGaag -5' |
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23614 | 5' | -55.5 | NC_005261.1 | + | 14149 | 0.66 | 0.897799 |
Target: 5'- gCGaGGACGCUCcagccgcgaacgGCGCGCGucucggggcgcGGGUCa-- -3' miRNA: 3'- -GCaUCUGCGAG------------UGCGCGU-----------CCCAGaag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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