Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23615 | 3' | -53.9 | NC_005261.1 | + | 125402 | 0.69 | 0.867037 |
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Target: 5'- -gUACGUUCCGGagCGgcGcgGGCGGCg -3' miRNA: 3'- uuGUGCAGGGCCa-GCauCuaCUGCUG- -5' |
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| 23615 | 3' | -53.9 | NC_005261.1 | + | 123683 | 0.69 | 0.859319 |
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Target: 5'- cGCACGUCCCGGcgcucagCGUGGGccGCGuACu -3' miRNA: 3'- uUGUGCAGGGCCa------GCAUCUacUGC-UG- -5' |
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| 23615 | 3' | -53.9 | NC_005261.1 | + | 121048 | 0.71 | 0.771659 |
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Target: 5'- cACACGUCgCGGUUGUGGcgcgcgguGUGcGCGGCg -3' miRNA: 3'- uUGUGCAGgGCCAGCAUC--------UAC-UGCUG- -5' |
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| 23615 | 3' | -53.9 | NC_005261.1 | + | 86534 | 0.75 | 0.50661 |
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Target: 5'- cAGCGCGgguUCCCGGccaCGUAGggGGCGGCg -3' miRNA: 3'- -UUGUGC---AGGGCCa--GCAUCuaCUGCUG- -5' |
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| 23615 | 3' | -53.9 | NC_005261.1 | + | 53255 | 0.76 | 0.458303 |
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Target: 5'- cGCGCGUCCCGGUCGcAGAccagcaUGuCGAUg -3' miRNA: 3'- uUGUGCAGGGCCAGCaUCU------ACuGCUG- -5' |
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| 23615 | 3' | -53.9 | NC_005261.1 | + | 138028 | 0.78 | 0.386513 |
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Target: 5'- cGGCGCGgCCCGG-CGgcGGUGGCGGCg -3' miRNA: 3'- -UUGUGCaGGGCCaGCauCUACUGCUG- -5' |
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| 23615 | 3' | -53.9 | NC_005261.1 | + | 357 | 0.78 | 0.386513 |
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Target: 5'- cGGCGCGgCCCGG-CGgcGGUGGCGGCg -3' miRNA: 3'- -UUGUGCaGGGCCaGCauCUACUGCUG- -5' |
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| 23615 | 3' | -53.9 | NC_005261.1 | + | 81562 | 1.08 | 0.004653 |
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Target: 5'- gAACACGUCCCGGUCGUAGAUGACGACg -3' miRNA: 3'- -UUGUGCAGGGCCAGCAUCUACUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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