Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23617 | 3' | -59.8 | NC_005261.1 | + | 6233 | 0.66 | 0.75213 |
Target: 5'- cCAGGUGCG-C-CCGGGgagggccugcCCGCCCcGCGg -3' miRNA: 3'- -GUCCAUGCaGaGGUCC----------GGCGGGcUGU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 40856 | 0.66 | 0.75213 |
Target: 5'- aGGGcGCGUCcgCgaAGGCCGCCCcgaacaggguucGACAg -3' miRNA: 3'- gUCCaUGCAGa-Gg-UCCGGCGGG------------CUGU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 115841 | 0.66 | 0.736923 |
Target: 5'- uGGGcGCGUUggcCCgcgcggcccccgccgAGGCgCGCCCGGCAa -3' miRNA: 3'- gUCCaUGCAGa--GG---------------UCCG-GCGGGCUGU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 101986 | 0.66 | 0.7244 |
Target: 5'- cCAGGUACGUCacggcgucguaguggCgCAGcucgccGCCGCCCGAg- -3' miRNA: 3'- -GUCCAUGCAGa--------------G-GUC------CGGCGGGCUgu -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 99831 | 0.66 | 0.703907 |
Target: 5'- cCGGGU-CGUCcgCCccGCCGCCCaGCGg -3' miRNA: 3'- -GUCCAuGCAGa-GGucCGGCGGGcUGU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 15352 | 0.66 | 0.703907 |
Target: 5'- cCAGGggaaagACGUC-CCGGcGgCGCUCGGCGc -3' miRNA: 3'- -GUCCa-----UGCAGaGGUC-CgGCGGGCUGU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 29979 | 0.66 | 0.703907 |
Target: 5'- -cGGUGCGcCgCC-GGCgCGUCCGGCAg -3' miRNA: 3'- guCCAUGCaGaGGuCCG-GCGGGCUGU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 64691 | 0.67 | 0.694054 |
Target: 5'- cCGGGcGCGUCcCgGGGCCGCCgCcGCGc -3' miRNA: 3'- -GUCCaUGCAGaGgUCCGGCGG-GcUGU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 35950 | 0.67 | 0.68415 |
Target: 5'- cCAGGccCGagcccggCCAGGCCGCUCGGCc -3' miRNA: 3'- -GUCCauGCaga----GGUCCGGCGGGCUGu -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 42900 | 0.67 | 0.68415 |
Target: 5'- -cGGcACGUgacccggcgCUCCgGGGCCGCCgGGCGa -3' miRNA: 3'- guCCaUGCA---------GAGG-UCCGGCGGgCUGU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 48743 | 0.67 | 0.68415 |
Target: 5'- gCAGGcacUGCGcguagUCgcgccccCCGGGCgGCCCGACGa -3' miRNA: 3'- -GUCC---AUGC-----AGa------GGUCCGgCGGGCUGU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 46677 | 0.67 | 0.674206 |
Target: 5'- -uGGccGCGcgCUCCAcggcGGCCGCCaCGGCAu -3' miRNA: 3'- guCCa-UGCa-GAGGU----CCGGCGG-GCUGU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 33917 | 0.67 | 0.674206 |
Target: 5'- gCGGGgccGCGUCUCUuGuGUCGCCCaGCAg -3' miRNA: 3'- -GUCCa--UGCAGAGGuC-CGGCGGGcUGU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 106837 | 0.67 | 0.664228 |
Target: 5'- -uGGUgcGCGUCgUCCAGcccGCCGCCUGGgGg -3' miRNA: 3'- guCCA--UGCAG-AGGUC---CGGCGGGCUgU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 19357 | 0.67 | 0.664228 |
Target: 5'- gGGGgaguuCGUCUCCAGGgUGCCagccaGGCc -3' miRNA: 3'- gUCCau---GCAGAGGUCCgGCGGg----CUGu -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 58366 | 0.67 | 0.654225 |
Target: 5'- cCAGGUgugcggcgACGUCggccUCCAGGgcgaacCCGCCCGGgAa -3' miRNA: 3'- -GUCCA--------UGCAG----AGGUCC------GGCGGGCUgU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 95369 | 0.67 | 0.644206 |
Target: 5'- gGGGUACuUCUCgC-GGCCcCCCGGCGc -3' miRNA: 3'- gUCCAUGcAGAG-GuCCGGcGGGCUGU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 31428 | 0.67 | 0.644206 |
Target: 5'- cCGGGgagGCG-CUggCGGcGCCGCCCGGCGa -3' miRNA: 3'- -GUCCa--UGCaGAg-GUC-CGGCGGGCUGU- -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 127583 | 0.68 | 0.634178 |
Target: 5'- cCAGGgcgcccuggGCGUCUUCgGGGCCGCgCUGAa- -3' miRNA: 3'- -GUCCa--------UGCAGAGG-UCCGGCG-GGCUgu -5' |
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23617 | 3' | -59.8 | NC_005261.1 | + | 122332 | 0.68 | 0.614123 |
Target: 5'- uCAGGUgGCG-CUCgCAGaGCuCGCCUGGCGg -3' miRNA: 3'- -GUCCA-UGCaGAG-GUC-CG-GCGGGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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