miRNA: 3'- aCAaGCGGUcCUCGCGCAUC-CGcaac -5'
miRNA: 3'- aCAa-GCGG--UCCuCGCGCAUCCGcaac -5'
miRNA: 3'- aCAAG----------CGGUCCUCGCGcaUCCGCaaC- -5'
miRNA: 3'- aCaa--GCGGUCCU---CGCGCa-----UCCGCaac -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23625 | 5' | -56.3 | NC_005261.1 | + | 123995 | 0.68 | 0.796288 |
|
Target: 5'- gGUaCGCCGaGAGCGCGUAGcGCu--- -3' miRNA: 3'- aCAaGCGGUcCUCGCGCAUC-CGcaac -5' |
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| 23625 | 5' | -56.3 | NC_005261.1 | + | 124807 | 0.69 | 0.729451 |
|
Target: 5'- cGUcgCGCUcuAGGuGCGCGUAGGCc--- -3' miRNA: 3'- aCAa-GCGG--UCCuCGCGCAUCCGcaac -5' |
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| 23625 | 5' | -56.3 | NC_005261.1 | + | 126930 | 0.69 | 0.709442 |
|
Target: 5'- cGUUCcagcagcgcgGCCAGGGGCGCc-GGGCGcgGg -3' miRNA: 3'- aCAAG----------CGGUCCUCGCGcaUCCGCaaC- -5' |
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| 23625 | 5' | -56.3 | NC_005261.1 | + | 127645 | 0.67 | 0.805306 |
|
Target: 5'- cGgcggCGCCGGGGggcGCGCGgguucgGGGCGg-- -3' miRNA: 3'- aCaa--GCGGUCCU---CGCGCa-----UCCGCaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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