miRNA: 3'- aCaaGCGGUCCuCGCGCA---------------UCCGCAac -5'
miRNA: 3'- acaaGCggUCCUCGCGCAUCCGCAac -5'
miRNA: 3'- aCAAGCGGUCC---UCGCgCAUC-CGCaac -5'
miRNA: 3'- -ACAAGCGGUCCUCGCGCAUCCGCAAC- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23625 | 5' | -56.3 | NC_005261.1 | + | 46824 | 0.74 | 0.452608 |
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Target: 5'- cGcgCGCCAGGcGCGCGUgcucgggaacgagguGGGCGUa- -3' miRNA: 3'- aCaaGCGGUCCuCGCGCA---------------UCCGCAac -5' |
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| 23625 | 5' | -56.3 | NC_005261.1 | + | 40721 | 0.74 | 0.439752 |
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Target: 5'- ---gCGagGGGGGCGCGUGGGCGUa- -3' miRNA: 3'- acaaGCggUCCUCGCGCAUCCGCAac -5' |
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| 23625 | 5' | -56.3 | NC_005261.1 | + | 64478 | 0.75 | 0.404206 |
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Target: 5'- gGUUCGCCGGGugcAGCGgGUGGcGCGg-- -3' miRNA: 3'- aCAAGCGGUCC---UCGCgCAUC-CGCaac -5' |
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| 23625 | 5' | -56.3 | NC_005261.1 | + | 73076 | 1.07 | 0.002878 |
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Target: 5'- gUGUUCGCCAGGAGCGCGUAGGCGUUGc -3' miRNA: 3'- -ACAAGCGGUCCUCGCGCAUCCGCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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