Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23645 | 5' | -51.3 | NC_005261.1 | + | 39242 | 0.66 | 0.988838 |
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Target: 5'- -cGGCGagGUCuccGGC-GAGGUCUCCa -3' miRNA: 3'- acUCGCagUAGu--UCGaCUUCAGAGGc -5' |
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| 23645 | 5' | -51.3 | NC_005261.1 | + | 12796 | 0.66 | 0.983761 |
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Target: 5'- cGAGCucGUCGUCGGcgucGCUGccGUcCUCCGc -3' miRNA: 3'- aCUCG--CAGUAGUU----CGACuuCA-GAGGC- -5' |
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| 23645 | 5' | -51.3 | NC_005261.1 | + | 40223 | 0.67 | 0.979512 |
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Target: 5'- aUGAGCGUCGUCGucucuGGCccGggGg--CCGu -3' miRNA: 3'- -ACUCGCAGUAGU-----UCGa-CuuCagaGGC- -5' |
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| 23645 | 5' | -51.3 | NC_005261.1 | + | 129681 | 0.67 | 0.979512 |
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Target: 5'- gGGGCGUCGcgacUCGGGCcGggGcCggggCCGg -3' miRNA: 3'- aCUCGCAGU----AGUUCGaCuuCaGa---GGC- -5' |
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| 23645 | 5' | -51.3 | NC_005261.1 | + | 39023 | 0.67 | 0.968622 |
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Target: 5'- aGAGCGUCcgCGAGC---AG-CUCCa -3' miRNA: 3'- aCUCGCAGuaGUUCGacuUCaGAGGc -5' |
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| 23645 | 5' | -51.3 | NC_005261.1 | + | 56471 | 0.68 | 0.965355 |
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Target: 5'- -cAGCGUCAUgGAggcGCUGGAGUCUa-- -3' miRNA: 3'- acUCGCAGUAgUU---CGACUUCAGAggc -5' |
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| 23645 | 5' | -51.3 | NC_005261.1 | + | 107277 | 0.68 | 0.958119 |
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Target: 5'- cGGGCGcgCAUCAGGCgGcAGgcaCUCCGc -3' miRNA: 3'- aCUCGCa-GUAGUUCGaCuUCa--GAGGC- -5' |
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| 23645 | 5' | -51.3 | NC_005261.1 | + | 108456 | 0.68 | 0.94991 |
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Target: 5'- aGcGCGUCGUC-GGC-GAGGaUCUCCGc -3' miRNA: 3'- aCuCGCAGUAGuUCGaCUUC-AGAGGC- -5' |
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| 23645 | 5' | -51.3 | NC_005261.1 | + | 67992 | 0.72 | 0.821759 |
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Target: 5'- cGcAGCGUCGUCGcGCacaacGggGUCUCCu -3' miRNA: 3'- aC-UCGCAGUAGUuCGa----CuuCAGAGGc -5' |
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| 23645 | 5' | -51.3 | NC_005261.1 | + | 54603 | 1.1 | 0.005811 |
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Target: 5'- gUGAGCGUCAUCAAGCUGAAGUCUCCGa -3' miRNA: 3'- -ACUCGCAGUAGUUCGACUUCAGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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