Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23649 | 3' | -57.1 | NC_005261.1 | + | 109118 | 0.66 | 0.880888 |
Target: 5'- aUGAGGgCGGCGGUGucGugCG-CGg -3' miRNA: 3'- gACUCCgGCUGCCACuaCugGCaGCa -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 82635 | 0.66 | 0.880888 |
Target: 5'- -cGAGGCCGACauGUGGUGGUCGUa-- -3' miRNA: 3'- gaCUCCGGCUGc-CACUACUGGCAgca -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 12760 | 0.66 | 0.880888 |
Target: 5'- -cGAGGUcguaggCGGCGGUGGcggggGGCuCGUCGg -3' miRNA: 3'- gaCUCCG------GCUGCCACUa----CUG-GCAGCa -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 16201 | 0.66 | 0.873798 |
Target: 5'- -gGGGGCCG-C-GUGAUGGCC-UCGa -3' miRNA: 3'- gaCUCCGGCuGcCACUACUGGcAGCa -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 26231 | 0.66 | 0.866495 |
Target: 5'- -gGGGGCa-GCGGUGAUaaGACCG-CGg -3' miRNA: 3'- gaCUCCGgcUGCCACUA--CUGGCaGCa -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 104919 | 0.66 | 0.858986 |
Target: 5'- --aGGGCCG-CGGUGAUGuuuGCCG-CGc -3' miRNA: 3'- gacUCCGGCuGCCACUAC---UGGCaGCa -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 48805 | 0.66 | 0.858986 |
Target: 5'- gUGAGGCCGugcGCGGcGuccgcgacGCCGUCGUc -3' miRNA: 3'- gACUCCGGC---UGCCaCuac-----UGGCAGCA- -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 61567 | 0.66 | 0.851276 |
Target: 5'- uCUGAuGGUggCGcUGGUGAUGGCCGuguUCGUg -3' miRNA: 3'- -GACU-CCG--GCuGCCACUACUGGC---AGCA- -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 28657 | 0.67 | 0.835278 |
Target: 5'- -cGAGGCCGGCGG----GGCCGaCGa -3' miRNA: 3'- gaCUCCGGCUGCCacuaCUGGCaGCa -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 5504 | 0.67 | 0.818555 |
Target: 5'- gCUGGGGCCGcggcagcgGCGGcGA-GGCCGcCGg -3' miRNA: 3'- -GACUCCGGC--------UGCCaCUaCUGGCaGCa -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 17649 | 0.67 | 0.801168 |
Target: 5'- -cGAGGCCGAUGaG-GAUGGCgCGccgCGUg -3' miRNA: 3'- gaCUCCGGCUGC-CaCUACUG-GCa--GCA- -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 23591 | 0.69 | 0.726312 |
Target: 5'- aUGGcGGCgG-UGGUGGUGGCUGUCGc -3' miRNA: 3'- gACU-CCGgCuGCCACUACUGGCAGCa -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 31462 | 0.7 | 0.656471 |
Target: 5'- -cGAGcGCCGGCGGccUGAgUGGCCGcCGg -3' miRNA: 3'- gaCUC-CGGCUGCC--ACU-ACUGGCaGCa -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 44882 | 0.7 | 0.626082 |
Target: 5'- -cGAGcGCCG-CGGgGAUGGCCGgggCGUa -3' miRNA: 3'- gaCUC-CGGCuGCCaCUACUGGCa--GCA- -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 28831 | 0.71 | 0.585659 |
Target: 5'- -cGAGGCgGccGCGGcUGAggcGGCCGUCGUg -3' miRNA: 3'- gaCUCCGgC--UGCC-ACUa--CUGGCAGCA- -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 126693 | 0.72 | 0.555662 |
Target: 5'- -cGAGGCCGGCGGUGcgGGaaG-CGg -3' miRNA: 3'- gaCUCCGGCUGCCACuaCUggCaGCa -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 85149 | 0.72 | 0.545758 |
Target: 5'- gUGAGGuuGACGuaguUGAUGGCCG-CGUa -3' miRNA: 3'- gACUCCggCUGCc---ACUACUGGCaGCA- -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 74807 | 0.73 | 0.497204 |
Target: 5'- -cGAGGCCcgcguGGCGGaGGUGGCCGcCGUg -3' miRNA: 3'- gaCUCCGG-----CUGCCaCUACUGGCaGCA- -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 70881 | 0.73 | 0.469017 |
Target: 5'- -cGuGGCCGGCGGUGcacAUGGCCGUg-- -3' miRNA: 3'- gaCuCCGGCUGCCAC---UACUGGCAgca -5' |
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23649 | 3' | -57.1 | NC_005261.1 | + | 49745 | 1.08 | 0.002855 |
Target: 5'- gCUGAGGCCGACGGUGAUGACCGUCGUg -3' miRNA: 3'- -GACUCCGGCUGCCACUACUGGCAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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