Results 1 - 20 of 67 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23655 | 3' | -56.9 | NC_005261.1 | + | 58289 | 0.66 | 0.850565 |
Target: 5'- gGCCGGCGcGCCGGGgggcGCgGGGAGGgggCg -3' miRNA: 3'- -CGGUUGU-UGGUCC----CGgUCCUCCacaG- -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 8 | 0.66 | 0.849768 |
Target: 5'- cGCCccuCGugCAgcGGGCCccgcgcgaccccgGGGGGGUGUUu -3' miRNA: 3'- -CGGuu-GUugGU--CCCGG-------------UCCUCCACAG- -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 64983 | 0.66 | 0.842506 |
Target: 5'- cGCCGcagcGCGugCAGGugcuCCAGGAGGUc-- -3' miRNA: 3'- -CGGU----UGUugGUCCc---GGUCCUCCAcag -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 74032 | 0.66 | 0.842506 |
Target: 5'- cCCAGCAagauggACCAGcGcGCCGGGGGGcUG-Cg -3' miRNA: 3'- cGGUUGU------UGGUC-C-CGGUCCUCC-ACaG- -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 137692 | 0.66 | 0.842506 |
Target: 5'- cGCCccuCGugCAgcGGGCCccgcgcgaccccGGGGGGUGUUu -3' miRNA: 3'- -CGGuu-GUugGU--CCCGG------------UCCUCCACAG- -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 27368 | 0.66 | 0.842506 |
Target: 5'- aCCGGCGcgagGCCGGuGGgCAGGGGGUa-- -3' miRNA: 3'- cGGUUGU----UGGUC-CCgGUCCUCCAcag -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 43000 | 0.66 | 0.834253 |
Target: 5'- gGCU-AC-ACCgAGGGCCAGGGcGUGUa -3' miRNA: 3'- -CGGuUGuUGG-UCCCGGUCCUcCACAg -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 28245 | 0.66 | 0.825814 |
Target: 5'- cGCgCGAUAGCgGGGGCUgcucgGGGGGGcgcUGUUg -3' miRNA: 3'- -CG-GUUGUUGgUCCCGG-----UCCUCC---ACAG- -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 111450 | 0.66 | 0.825814 |
Target: 5'- aCCGGCgAGCCGGGcGCCGGcGAGGc--- -3' miRNA: 3'- cGGUUG-UUGGUCC-CGGUC-CUCCacag -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 31546 | 0.66 | 0.825814 |
Target: 5'- cGCCGACucgcacGCCuGGGCCGGGuccuGGa--- -3' miRNA: 3'- -CGGUUGu-----UGGuCCCGGUCCu---CCacag -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 52983 | 0.66 | 0.825814 |
Target: 5'- cGCCGGCAGgCGGGGgCGGcGcccAGGcGUCg -3' miRNA: 3'- -CGGUUGUUgGUCCCgGUC-C---UCCaCAG- -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 1279 | 0.66 | 0.817196 |
Target: 5'- uGCCGGCGcCCA-GGCCGGcGGGGcucccGUCg -3' miRNA: 3'- -CGGUUGUuGGUcCCGGUC-CUCCa----CAG- -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 97350 | 0.67 | 0.808407 |
Target: 5'- aGCCAAUGACCguGGGGUCuacggcccGGGcGUGUCg -3' miRNA: 3'- -CGGUUGUUGG--UCCCGGu-------CCUcCACAG- -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 29001 | 0.67 | 0.808407 |
Target: 5'- cGCCGACu-CCGGGGCCGaugccaaagacGcGAGG-GUUg -3' miRNA: 3'- -CGGUUGuuGGUCCCGGU-----------C-CUCCaCAG- -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 107409 | 0.67 | 0.808407 |
Target: 5'- cCCGGCcGCCAGGGCCGaGAGcuucGUCc -3' miRNA: 3'- cGGUUGuUGGUCCCGGUcCUCca--CAG- -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 109110 | 0.67 | 0.808407 |
Target: 5'- cGCCGGCcAUgAGGGC--GGcGGUGUCg -3' miRNA: 3'- -CGGUUGuUGgUCCCGguCCuCCACAG- -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 101732 | 0.67 | 0.807519 |
Target: 5'- cGCCcGCGAUCAGGGCCucgcucGGcagcuccAGGUG-Ca -3' miRNA: 3'- -CGGuUGUUGGUCCCGGu-----CC-------UCCACaG- -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 98676 | 0.67 | 0.799456 |
Target: 5'- gGCgGGCuccGCCGGGGCC-GGAGGc--- -3' miRNA: 3'- -CGgUUGu--UGGUCCCGGuCCUCCacag -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 38595 | 0.67 | 0.799456 |
Target: 5'- uGCUGGCGGCCcGcGGCUGGcGGGUGUUg -3' miRNA: 3'- -CGGUUGUUGGuC-CCGGUCcUCCACAG- -5' |
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23655 | 3' | -56.9 | NC_005261.1 | + | 20265 | 0.67 | 0.790352 |
Target: 5'- cGCCGAgAACC---GCCGGGucGUGUCg -3' miRNA: 3'- -CGGUUgUUGGuccCGGUCCucCACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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