Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2366 | 3' | -53 | NC_001416.1 | + | 44533 | 0.66 | 0.809614 |
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Target: 5'- cCAUCCGUGGCaaUCAUGCUgcuacguGUGAc -3' miRNA: 3'- -GUAGGCACCGa-GGUGCGGuuuu---CACU- -5' |
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| 2366 | 3' | -53 | NC_001416.1 | + | 14184 | 0.66 | 0.779564 |
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Target: 5'- -cUUCGUGGUaagCACGCCGGAGGggGAa -3' miRNA: 3'- guAGGCACCGag-GUGCGGUUUUCa-CU- -5' |
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| 2366 | 3' | -53 | NC_001416.1 | + | 9489 | 0.67 | 0.70435 |
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Target: 5'- --aCCGUGGCcuUCCA-GCCGGAGGg-- -3' miRNA: 3'- guaGGCACCG--AGGUgCGGUUUUCacu -5' |
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| 2366 | 3' | -53 | NC_001416.1 | + | 9261 | 0.74 | 0.330731 |
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Target: 5'- -cUCCG-GGCaUCCACGCCAGc-GUGAa -3' miRNA: 3'- guAGGCaCCG-AGGUGCGGUUuuCACU- -5' |
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| 2366 | 3' | -53 | NC_001416.1 | + | 844 | 0.76 | 0.255481 |
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Target: 5'- gGUCUGUGGCUCCG-GCCuGAacAGUGAg -3' miRNA: 3'- gUAGGCACCGAGGUgCGGuUU--UCACU- -5' |
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| 2366 | 3' | -53 | NC_001416.1 | + | 1335 | 1.09 | 0.001296 |
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Target: 5'- cCAUCCGUGGCUCCACGCCAAAAGUGAg -3' miRNA: 3'- -GUAGGCACCGAGGUGCGGUUUUCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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